School of Clinical Stomatology, Tianjin Medical University, Tianjin, 300070, China; Department of Orthodontics, Tianjin Stomatological Hospital, Tianjin, 300041, China; Tianjin Key Laboratory of Oral and Maxillofacial Function Reconstruction, 75 Dagu Road, Heping District, Tianjin, 300041, China.
Department of Oral and Maxillofacial Surgery, Tianjin Stomatological Hospital, Tianjin, 300041, China; Tianjin Key Laboratory of Oral and Maxillofacial Function Reconstruction, 75 Dagu Road, Heping District, Tianjin, 300041, China.
Exp Cell Res. 2022 Oct 15;419(2):113318. doi: 10.1016/j.yexcr.2022.113318. Epub 2022 Aug 15.
Several studies have confirmed that exosomes containing microRNAs (miRNAs) from the aseptic inflammatory microenvironment play an important role in bone remodeling. But the mechanism that induces changes in the osteogenic ability of periodontal ligament stem cells (PDLSCs) is still unclear. In the present study, the osteogenic function of periodontal ligament fibroblasts-derived exosomes induced by PGE on PDLSCs was detected by real-time PCR, alizarin red assay and alkaline phosphatase staining. High-throughput miRNAs sequencing was used to reveal that miR-34c-5p in exosomes-PGE was upregulated compared it in exosomes-normal. Real-time PCR and western blotting assay verified that overexpression of miR-34c-5p inhibited osteogenic differentiation, and reduced phosphorylation of ERK1/2. In addition, dual-luciferase reporter assay revealed that miR-34c-5p targeted special AT-rich sequence-binding protein 2 (SATB2). It was shown that exosomal miR-34c-5p inhibited osteogenic differentiation of PDLSCs via SATB2/ERK pathway.
多项研究证实，来自无菌性炎症微环境的含有 microRNAs (miRNAs) 的外泌体在骨重塑中发挥重要作用。但诱导牙周膜干细胞 (PDLSCs) 成骨能力变化的机制尚不清楚。本研究通过实时 PCR、茜素红测定和碱性磷酸酶染色检测 PGE 诱导的牙周膜成纤维细胞衍生的外泌体对 PDLSCs 的成骨功能。高通量 miRNAs 测序表明，外泌体-PGE 中的 miR-34c-5p 上调，而外泌体-正常中的 miR-34c-5p 下调。实时 PCR 和 Western blot 检测验证了 miR-34c-5p 的过表达抑制了成骨分化，并降低了 ERK1/2 的磷酸化。此外，双荧光素酶报告基因检测表明，miR-34c-5p 靶向特殊富含 AT 的序列结合蛋白 2 (SATB2)。结果表明，外泌体 miR-34c-5p 通过 SATB2/ERK 通路抑制 PDLSCs 的成骨分化。
