Reik L M, Maines S L, Ryan D E, Levin W, Bandiera S, Thomas P E
J Immunol Methods. 1987 Jun 26;100(1-2):123-30. doi: 10.1016/0022-1759(87)90180-3.
A simple, non-chromatographic purification procedure for monoclonal antibodies from mouse ascites fluid is described. This procedure, which is rapid, inexpensive, and has high capacity involves the precipitation of contaminating proteins with caprylic acid followed by precipitation of immunoglobulin using ammonium sulfate. This two-step procedure is shown to be effective for the purification of various immunoglobulins including IgG1, IgG2a and IgG2b. In the present report, more than 30 monoclonal antibodies directed against cytochrome P450 isozymes have been purified by this method and characterized.
本文描述了一种从小鼠腹水中纯化单克隆抗体的简单非色谱纯化方法。该方法快速、廉价且容量大，包括先用辛酸沉淀污染蛋白，然后用硫酸铵沉淀免疫球蛋白。结果表明，这种两步法对于纯化包括IgG1、IgG2a和IgG2b在内的各种免疫球蛋白均有效。在本报告中，已有30多种针对细胞色素P450同工酶的单克隆抗体通过该方法得到纯化和鉴定。
