Department of Biological and Biochemical Sciences, University of Pardubice, Pardubice, Czech Republic.
Department of Environmental Microbiology and Biotechnology, Nicolaus Copernicus University in Toruń, Torun, Poland.
J Appl Microbiol. 2022 Oct;133(4):2255-2266. doi: 10.1111/jam.15786. Epub 2022 Aug 31.
The purpose of the study was to evaluate the occurrence of Yersinia enterocolitica in different types of wastewater and to characterize the isolates by biotyping, serotyping, and antimicrobial susceptibility testing. In addition, cultivation protocols were evaluated.
The occurrence of Y. enterocolitica was determined in treated and untreated municipal wastewater, as well as in hospital, slaughterhouse, and cowshed wastewater. Y. enterocolitica was detected in 84.1% of the wastewater samples, while the main sources were untreated municipal and slaughterhouse wastewater. In contrast, the lowest incidence was found in hospital wastewater. An exclusive occurrence of biotype 1A (98.3%) was detected. Pathogenic bio-serotypes 4/O:3 and 3/O:3 were isolated only from slaughterhouse wastewater. The highest resistance rates were observed for ampicillin (92.5%) and amoxicillin-clavulanic acid (36.8%).
Y. enterocolitica was commonly detected in wastewater, although the prevalence varied depending on the origin of the wastewater. No single cultivation protocol was able to recover Y. enterocolitica isolates from such a complex matrix as wastewater.
Our study provided data that can contribute to the evaluation of wastewater as a source of Y. enterocolitica and to understanding the threat of wastewater isolates to human health.
本研究旨在评估不同类型废水中耶尔森氏菌属肠结肠炎的发生情况，并通过生物分型、血清分型和抗菌药物敏感性测试对分离株进行特征描述。此外，还评估了培养方案。
本研究检测了处理过和未处理的城市废水以及医院、屠宰场和牛舍废水中耶尔森氏菌属肠结肠炎的发生情况。研究结果显示，84.1%的废水样本中检测到了耶尔森氏菌属肠结肠炎，而主要来源是未经处理的城市和屠宰场废水。相比之下，医院废水的检出率最低。检测到的生物型均为 1A 型（98.3%）。仅从屠宰场废水中分离出了致病性生物血清型 4/O:3 和 3/O:3。对氨苄西林（92.5%）和阿莫西林克拉维酸（36.8%）的耐药率最高。
尽管废水的来源不同，但耶尔森氏菌属肠结肠炎在废水中普遍存在。没有单一的培养方案能够从如此复杂的废水基质中回收耶尔森氏菌属肠结肠炎分离株。
本研究提供了数据，可以帮助评估废水作为耶尔森氏菌属肠结肠炎的来源，并了解废水分离株对人类健康的威胁。
