Huizenga J R, Gips C H
J Clin Chem Clin Biochem. 1987 Mar;25(3):161-5. doi: 10.1515/cclm.1987.25.3.161.
A pseudocholinesterase catalytic activity assay using p-hydroxybenzoylcholine as substrate and measuring the decrease of NADPH at 340 nm was compared with a colorimetric method using acetylthiocholine as substrate. The assay is simple, uses 50 microliters serum and is performed at 37 degrees C. Precision of the UV-340 method was good except at low ranges. The catalytic activity was depressed by the anticoagulants citrate and fluoride but not by EDTA or heparin. The reference values obtained with the evaluated UV-340 method are somewhat higher than those with the colorimetric method. As the results with both methods are comparable, the choice of procedure will depend on the local facilities.
将以对羟基苯甲酰胆碱为底物、通过测量340nm处NADPH的减少来进行的假胆碱酯酶催化活性测定法，与以乙酰硫代胆碱为底物的比色法进行了比较。该测定法操作简单，使用50微升血清，且在37摄氏度下进行。除了低浓度范围外，UV-340法的精密度良好。抗凝剂柠檬酸盐和氟化物会降低催化活性，但乙二胺四乙酸（EDTA）或肝素不会。用评估的UV-340法获得的参考值略高于比色法的参考值。由于两种方法的结果具有可比性，操作方法的选择将取决于当地的设备条件。
