De Gennaro L D, Packard D S, Stach R W, Wagner B J
Growth. 1980 Dec;44(4):343-54.
The growth and differentiation of chicken embryos maintained in evaporating dishes as shell-less cultures were compared to that of control embryos in ovo under the same conditions of incubation. The development of cultured embryos appears to be indistinguishable from that of embryos in ovo up to 10 days of total incubation. Thereafter, the growth of shell-less embryos is significantly retarded as evidenced by morphological criteria of the Hamburger-Hamilton stages of development, body weight, tibia length, and total protein content. Cultured embryos are smaller in size than controls, but show normal morphological features including a fully-differentiated skeleton. Skeletal defects and other anomalies were not detected in any of the specimens studied. Limitations as to the use of the simplified method of culture are evaluated and discussed.
将置于蒸发皿中作为无壳培养物的鸡胚生长和分化情况，与在相同孵化条件下处于蛋内的对照胚胎进行了比较。在整个孵化10天之前，培养胚胎的发育与蛋内胚胎的发育似乎没有区别。此后，根据汉堡-汉密尔顿发育阶段的形态学标准、体重、胫骨长度和总蛋白含量来看，无壳胚胎的生长明显迟缓。培养的胚胎比对照胚胎体积小，但显示出正常的形态特征，包括完全分化的骨骼。在所研究的任何标本中均未检测到骨骼缺陷和其他异常情况。对这种简化培养方法的使用局限性进行了评估和讨论。
