Ono T, Tuan R S
J Embryol Exp Morphol. 1986 Mar;92:207-22.
To study the effects of systemic calcium deficiency on embryonic development, chick embryos maintained in long-term shell-less cultures were compared to control embryos incubated in ovo with respect to various parameters of metabolism and growth. After incubation day 14, retarded growth and development were apparent in shell-less embryos which exhibited severe hypocalcaemia and hyperphosphataemia. A development-specific necrosis of the liver tissues was observed in both shell-less and control embryos, but the frequency and extent of tissue abnormality were significantly greater in the former. Serum levels of lactate dehydrogenase and alkaline phosphatase were considerably elevated in shell-less embryos. Electrophoretic analysis revealed that the relative levels of two major serum proteins were also altered in shell-less embryos.
为研究全身性钙缺乏对胚胎发育的影响，将长期在无壳培养条件下维持的鸡胚与在卵内孵化的对照胚胎在各种代谢和生长参数方面进行比较。在孵化第14天后，无壳胚胎中明显出现生长和发育迟缓，表现为严重的低钙血症和高磷血症。在无壳胚胎和对照胚胎中均观察到肝脏组织的发育特异性坏死，但前者组织异常的频率和程度明显更高。无壳胚胎中乳酸脱氢酶和碱性磷酸酶的血清水平显著升高。电泳分析显示，无壳胚胎中两种主要血清蛋白的相对水平也发生了改变。
