Ma Qianying, Shi Weizhi, Jahan Kifat, Zhao Wen, Xu Qiaoyue, Huo Zhongming, Fang Lei, Yang Feng, Yan Xiwu
Engineering Research Center of Shellfish Culture and Breeding in Liaoning Province, Dalian 116023, China; College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China.
Engineering Research Center of Shellfish Culture and Breeding in Liaoning Province, Dalian 116023, China; College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China.
Comp Biochem Physiol Part D Genomics Proteomics. 2022 Dec;44:101016. doi: 10.1016/j.cbd.2022.101016. Epub 2022 Jul 29.
In this research, we identified genes associated with ammonia nitrogen (TAN) stress response and resistance in juveniles of the Zebra II strain and a wild population of the Manila clam Ruditapes philippinarum. Both groups were subjected to a 96 h acute toxicity test using TAN concentrations of 17.617 ± 0.634 and 16.670 ± 0.7 mg/l, respectively. We then collected samples, conducted transcriptome sequencing and screened the sequences for differentially expressed genes (DEGs) related to TAN stress response. We identified 2908 and 2861 DEGs in the Zebra II and wild clam groups, respectively, and the two groups had 626 DEGs in common. The verified DEGs had less of a detoxification effect in the wild population than that in the Zebra II group. Gene Ontology database analysis showed that Zebra II juveniles were mainly enriched in protein phosphorylation, purine nucleoside binding, and kinase activity, whereas the wild population juveniles were primarily enriched in oxidases activity, organic acid metabolic processes, and extracellular regions. Kyoto Encyclopedia of Genes and Genomes pathway analysis mainly highlighted aminoacyl tRNA biosynthesis in Zebra II juveniles and sphingolipid metabolism, FOXO signaling, biosynthesis of aminoacyl tRNA, and other pathways in the wild population. These results show that the toxic effect of TAN on the Manila clam is related to a variety of pathways, which are mainly related to immune response, inflammatory response, metabolic pathways, and nerve conduction. This study provides basic data and theoretical reference for revealing the molecular regulation mechanism of the improved TAN tolerance of Zebra II strain as compared with the wild population of Ruditapes philippinarum.
在本研究中，我们鉴定了与斑马二号品系菲律宾蛤仔幼体以及菲律宾蛤仔野生群体中氨氮（TAN）应激反应和抗性相关的基因。两组分别使用浓度为17.617±0.634和16.670±0.7mg/L的TAN进行96小时急性毒性试验。然后我们采集样本，进行转录组测序，并筛选出与TAN应激反应相关的差异表达基因（DEG）。我们在斑马二号和野生蛤仔群体中分别鉴定出2908个和2861个DEG，两组共有626个DEG。经验证的DEG在野生群体中的解毒作用比在斑马二号群体中弱。基因本体数据库分析表明，斑马二号幼体主要富集于蛋白质磷酸化、嘌呤核苷结合和激酶活性，而野生群体幼体主要富集于氧化酶活性、有机酸代谢过程和细胞外区域。京都基因与基因组百科全书通路分析主要突出了斑马二号幼体中的氨酰tRNA生物合成以及野生群体中的鞘脂代谢、FOXO信号传导、氨酰tRNA生物合成等通路。这些结果表明，TAN对菲律宾蛤仔的毒性作用与多种通路有关，主要与免疫反应、炎症反应、代谢通路和神经传导有关。本研究为揭示斑马二号品系与菲律宾蛤仔野生群体相比TAN耐受性提高的分子调控机制提供了基础数据和理论参考。
