Bioprocess Engineering Laboratory, Centre for Bioenergy, School of Chemical and Biotechnology, SASTRA Deemed to be University, Thanjavur, 613401, Tamil Nadu, India.
Bioprocess Engineering Laboratory, Centre for Bioenergy, School of Chemical and Biotechnology, SASTRA Deemed to be University, Thanjavur, 613401, Tamil Nadu, India.
Int J Biol Macromol. 2022 Nov 1;220:775-787. doi: 10.1016/j.ijbiomac.2022.08.094. Epub 2022 Aug 17.
This study focuses the comparison on yield of microbial dextran using treated sugarcane molasses (SCM) as a feed stock from different treatment methods. The suitable method for treatment of SCM was identified on the basis of microbial dextran production. The different factors namely the concentrations of total sugars, nitrogen sources, inoculum size, shaking speed, initial medium pH, and phosphate sources influencing the production of microbial dextran were studied. The maximum yield of dextran was obtained to be 17.18 ± 0.08 g L using the conventional optimization. The structural analysis of produced dextran from SCM with various treatment techniques was compared using Fourier-transform infra-red analysis and nuclear magnetic resonance spectroscopy. Later, the rheological behavior of produced microbial dextran was examined and found to be a non-Newtonian. To the best of our knowledge, the comparison on the production of microbial dextran through fermentation using SCM with various treatment strategies has not been performed yet.
本研究聚焦于使用不同处理方法的甘蔗废糖蜜（SCM）作为原料时微生物葡聚糖产量的比较。基于微生物葡聚糖的生产，确定了 SCM 的合适处理方法。研究了影响微生物葡聚糖生产的不同因素，即总糖浓度、氮源、接种量、摇床速度、初始培养基 pH 值和磷酸盐源。通过常规优化，葡聚糖的最大产量为 17.18±0.08 g/L。使用傅里叶变换红外分析和核磁共振波谱对不同处理技术生产的 SCM 葡聚糖的结构分析进行了比较。随后，对所生产的微生物葡聚糖的流变行为进行了研究，发现其为非牛顿流体。据我们所知，目前尚未有使用不同处理策略的 SCM 通过发酵生产微生物葡聚糖的比较研究。
