Chemical Engineering and Food Engineering Department, Federal University of Santa Catarina - UFSC, Florianópolis, SC, Zip Code 88040-900, Brazil.
Braz J Microbiol. 2021 Jun;52(2):587-596. doi: 10.1007/s42770-021-00456-9. Epub 2021 Mar 2.
Three culture media were studied for red pigment production by Monascus ruber in submerged cultivation: rice flour (20 g L), sugarcane molasses (30 g L), and, finally, molasses + rice flour (10 g L+10 g L); all culture media were added of 5 g L glycine as nitrogen source. Rice flour showed pigment production of 7.05 UA and molasses 5.08 UA, and the mixture of rice flour and molasses showed the best result of 16.38 UA Molasses culture presented good results for cell biomass production of 11.09 g L. With these results, it was observed that one substrate presented good pigment production (rice flour) and another attained better results for cell biomass growth (molasses), and a third medium containing 10 g L of rice flour + 10 g L of molasses was formulated. The results for this mixture showed satisfactory results, with global pigment productivity of 0.097 UA h and maximum productivity rate of 0.17 UA h. The high production and productivity obtained for the mixture of rice flour and molasses indicated that the production of red pigment by submerged fermentation, using the mixture of these low-cost culture media, may be promising in terms of commercial production.
研究了三种培养基用于红曲霉菌(Monascus ruber)的液体深层发酵生产红色素:米粉(20 g/L)、甘蔗糖蜜(30 g/L),最后是糖蜜+米粉(10 g/L+10 g/L);所有培养基均添加 5 g/L 甘氨酸作为氮源。米粉显示出 7.05 UA 的色素产量,糖蜜显示出 5.08 UA 的色素产量,而米粉和糖蜜的混合物显示出 16.38 UA 的最佳结果。糖蜜培养基对细胞生物量的生产表现出良好的效果,达到 11.09 g/L。根据这些结果,可以观察到一种基质具有良好的色素生产能力(米粉),另一种基质对细胞生物量的生长具有更好的效果(糖蜜),因此配制了一种含有 10 g/L 米粉和 10 g/L 糖蜜的第三种培养基。该混合物的结果令人满意,总色素生产率为 0.097 UA/h,最大生产率为 0.17 UA/h。米粉和糖蜜混合物的高产量和高生产率表明,使用这些低成本培养基的混合物进行液体深层发酵生产红色素,在商业生产方面可能具有很大的潜力。