环状 RNA circRara 促进米氏绒螯蟹先天免疫反应。
Circular RNA circRara promote the innate immune responses in miiuy croaker, Miichthys miiuy.
机构信息
Laboratory of Fish Molecular Immunology, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China.
Laboratory of Fish Molecular Immunology, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China; Laboratory of Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources (Shanghai Ocean University), Ministry of Education, 201306, China; National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, 201306, China.
出版信息
Fish Shellfish Immunol. 2022 Sep;128:557-564. doi: 10.1016/j.fsi.2022.08.026. Epub 2022 Aug 19.
With the in-depth study of circRNA, more and more biological studies have shown that circRNAs play an important role in mammals, such as cell proliferation, apoptosis, invasion, development and disease state. However, the regulatory mechanism of circRNA in lower vertebrates remains unclear. Here, we found a new circular RNA and named it circRara. We carried out the experimental study on its antiviral and antibacterial response, cell proliferation and activity. The results showed that circRara had a positive regulatory effect on the antiviral and antibacterial response, cell proliferation and activity in miiuy croaker. First, we found that the expression of circRara could be up-regulated under the stimulation of LPS and poly (I: C), but not the expression of linear Rara. In addition, the increase of circRara can increase the production of inflammatory factors and antiviral genes, which was confirmed by double luciferase reporter gene experiment and qPCR. These results will help to further understand the immunomodulatory mechanism of circRNA in teleost fish.
随着 circRNA 的深入研究，越来越多的生物学研究表明 circRNAs 在哺乳动物中发挥着重要作用，例如细胞增殖、凋亡、侵袭、发育和疾病状态。然而，circRNA 在低等脊椎动物中的调控机制尚不清楚。在这里，我们发现了一种新的环状 RNA，并将其命名为 circRara。我们对其抗病毒和抗菌反应、细胞增殖和活性进行了实验研究。结果表明，circRara 对米氏鲈的抗病毒和抗菌反应、细胞增殖和活性具有正向调节作用。首先，我们发现 circRara 的表达可以在 LPS 和 poly (I: C) 的刺激下上调，但线性 Rara 的表达不会上调。此外，circRara 的增加可以增加炎症因子和抗病毒基因的产生，这一点通过双荧光素酶报告基因实验和 qPCR 得到了证实。这些结果将有助于进一步了解 circRNA 在硬骨鱼类中的免疫调节机制。
Zotero 插件