Laboratory of Fish Molecular Immunology, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China.
Laboratory of Fish Molecular Immunology, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China; Laboratory of Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
Dev Comp Immunol. 2023 May;142:104653. doi: 10.1016/j.dci.2023.104653. Epub 2023 Feb 1.
In recent years, many studies have shown that long non-coding RNAs (lncRNAs) can regulate many biochemical processes, such as cell growth, proliferation, and immune response, which have attracted great attention. There are relatively many studies on lncRNA in mammals, while the research on lncRNA in lower vertebrates has just begun. In this study, we found a lncRNA, lncRNA LTCONS8875, related to innate immune response in Miichthys miiuy (miiuy croaker). Our results showed that lncRNA LTCONS8875 can up-regulate the expression of IRAK4 at the mRNA and protein levels, and significantly increase the production of inflammatory factors under LPS stimulation. Our research also confirmed that lncRNA LTCONS8875 plays an active role in regulating inflammation, cell proliferation, and cell viability. In summary, this research results showed that lncRNA LTCONS8875 can as an active regulatory role of innate immunity in miiuy croaker by up-regulating the expression of IRAK4, providing some insights for understanding the network mechanism of non-coding regulation of fish immunity.
近年来，许多研究表明长非编码 RNA（lncRNA）可以调节许多生化过程，如细胞生长、增殖和免疫反应，这引起了极大的关注。哺乳动物中关于 lncRNA 的研究相对较多，而对低等脊椎动物中的 lncRNA 的研究才刚刚开始。在这项研究中，我们在 Miichthys miiuy（米氏黄鱼）中发现了一个与先天免疫反应相关的 lncRNA，lncRNA LTCONS8875。我们的结果表明，lncRNA LTCONS8875 可以在 mRNA 和蛋白质水平上上调 IRAK4 的表达，并在 LPS 刺激下显著增加炎症因子的产生。我们的研究还证实，lncRNA LTCONS8875 在调节炎症、细胞增殖和细胞活力方面发挥着积极的作用。总之，这项研究结果表明，lncRNA LTCONS8875 通过上调 IRAK4 的表达，在米氏黄鱼中发挥着先天免疫的积极调节作用，为理解鱼类免疫的非编码调控网络机制提供了一些见解。
