School of Integrated Traditional Chinese and Western Medicine, Hebei University of Chinese Medicine, Hebei, P. R. China.
Hebei Traditional Chinese Medicine Formula Granule Engineering & Technology Innovate Center, Hebei University of Chinese Medicine, Hebei, P. R. China; Quality Evaluation & Standardization Hebei Province Engineering Research Center of Traditional Chinese Medicine, Hebei University of Chinese Medicine, Hebei, P. R. China; TCM Formula Granule Research Center of Hebei Province University, Hebei University of Chinese Medicine, Hebei, P. R. China.
J Sep Sci. 2022 Oct;45(20):3838-3851. doi: 10.1002/jssc.202200327.
Danggui Jianzhong decoction is a classical prescription that has been widely used for thousands of years. However, the quality of this formula is difficult to control owing to its complex chemical component system. In this study, a simple and efficient method comprising ultra-high-performance liquid chromatography fingerprint, chemical pattern recognition, and network pharmacology was established to evaluate the quality of this decoction. A total of 20 common peaks were obtained by fingerprint analysis and 19 chemicals were identified. The fingerprint similarity of 15 batch samples ranged from 0.963 to 0.991. Chemical pattern recognition analysis could clearly classify 15 batches of Danggui Jianzhong decoction into three groups. Further, seven chemical markers were screened out. A herbs-active components-targets-disease network was constructed and enrichment analyses were performed, which indicated that these 19 chemical components are the medicinal substances of Danggui Jianzhong decoction. Further, the mechanism employed by this formula to treat primary dysmenorrhea may be related to the regulation of inflammatory response. In conclusion, this combination approach enables accurate evaluation and prediction of the quality of Danggui Jianzhong decoction, and lays the foundation for studies on the material basis and exploration of the mechanism of Danggui Jianzhong decoction in the treatment of primary dysmenorrhea.
当归建中汤是一种经典的方剂，已有数千年的广泛应用。然而，由于其复杂的化学组成体系，该配方的质量难以控制。在这项研究中，建立了一种简单高效的方法，包括超高效液相色谱指纹图谱、化学模式识别和网络药理学，以评估该汤的质量。指纹图谱分析得到了 20 个共有峰，鉴定出 19 种化学成分。15 批样品的指纹相似度在 0.963 至 0.991 之间。化学模式识别分析可以清楚地将 15 批当归建中汤分为三组。进一步筛选出 7 个化学标志物。构建了一个草药-活性成分-靶点-疾病网络，并进行了富集分析，表明这 19 种化学成分是当归建中汤的药用物质。此外，该配方治疗原发性痛经的机制可能与炎症反应的调节有关。总之，这种组合方法能够准确评估和预测当归建中汤的质量，为研究当归建中汤治疗原发性痛经的物质基础和作用机制奠定了基础。
