Porter Megan L, Lutz Holly, Steck Mireille, Chong Rebecca A
School of Life Sciences, University of Hawai'i at Mānoa, Honolulu, HI, USA.
Department of Pediatrics, University of California San Diego, CA, USA.
Mitochondrial DNA B Resour. 2022 Aug 17;7(8):1486-1488. doi: 10.1080/23802359.2022.2107450. eCollection 2022.
We sequenced the complete mitochondrial genomes of two bat fly species within the Nycteribiidae (Diptera: Hippoboscoidea) - (Cyclopodiinae) and (Nycteribiinae). Both mitogenomes were complete and contained 13 protein-coding genes, 22 tRNAs, and two rRNAs. Relative to the inferred ancestral gene order of dipteran mitochondrial genomes, no rearrangements were identified in either species. There were large differences in size between the two genomes, with having a larger genome (19,164 bp) than (16,964 bp); both species had larger genomes than two previously published Streblidae bat fly species (e.g., and ). The increased genome sizes were due to expansions in the control region and the non-coding region downstream of the light-strand origin of replication. Additional differences between the two mitogenomes included a significantly longer cox3 gene in and a longer nad1 gene in . Interestingly, both genomes also had the lowest GC content ( - 15.9%; - 17.0%) of any available Hippoboscoidea mitochondrial genome (18.8-23.9%). These mitogenomes represent the first sequences from species within the bat fly family Nycteribiidae. The sequence data here will provide a foundation for continued studies of genome evolution more generally within obligate blood-feeding ectoparasites, and specifically for the bat flies as vectors of significant 'bat-associated' viruses and microorganisms.
我们对蝠蝇科（双翅目：虱蝇总科）两个物种的线粒体全基因组进行了测序，分别是（柔螨亚科）和（蝠蝇亚科）。两个线粒体基因组均完整，包含13个蛋白质编码基因、22个tRNA和2个rRNA。相对于推断的双翅目线粒体基因组祖先基因顺序，这两个物种均未发现重排现象。两个基因组在大小上有很大差异，基因组比基因组大（19,164 bp对16,964 bp）；这两个物种的基因组都比之前发表的两个蝠蝇科物种（如和）的基因组大。基因组大小的增加是由于控制区和轻链复制起点下游非编码区的扩展。两个线粒体基因组的其他差异包括基因组中的cox3基因明显更长，基因组中的nad1基因更长。有趣的是，这两个基因组的GC含量也是所有已知虱蝇总科线粒体基因组中最低的（基因组为15.9%；基因组为17.0%，而虱蝇总科线粒体基因组的GC含量为18.8 - 23.9%）。这些线粒体基因组代表了蝠蝇科物种的首批序列数据。这里的序列数据将为更广泛地持续研究专性吸血外寄生虫的基因组进化奠定基础，特别是为作为重要“蝙蝠相关”病毒和微生物载体的蝠蝇的研究奠定基础。
