Wu Fengyang, Gao Lijie, Li Fei, Cui Jia, Yang Xinyu, Liu Yanhua, Chen Saijuan, Chen Baojiang
College of Animal Science and Technology, Hebei Agricultural University, Baoding, China.
Hebei Provincial Animal Husbandry Station, Shijiazhuang, China.
Front Vet Sci. 2022 Aug 3;9:950063. doi: 10.3389/fvets.2022.950063. eCollection 2022.
This experiment aimed to establish the effects of zearalenone (ZEN) on ovarian development in prepubertal gilts through the growth hormone axis [growth hormone-releasing hormone (GHRH) / growth hormone (GH) / growth hormone receptor (GHR)]. In a 40-day experiment, 48 Landrace × Yorkshire crossbred prepubertal gilts were randomly allocated to four dietary treatments, including a basal diet supplemented with 0 (control), 400 (T1), 800 (T2), and 1,600 (T3) μg/kg ZEN. The ovary index of T2 ( = 0.058) and T3 ( = 0.065) increased compared to the control group. Besides, histopathological examination revealed that ZEN promoted the development of ovaries and follicles. The GHR content, relative expression levels of , janus activated kinase 2 () mRNA, and mean optical density of GHR in the ovaries of prepubertal gilts in the T2 experimental group increased significantly at < 0.05 compared to the control group. The T3 group had significantly higher GHR content, relative expression levels, and signal transducer and activator of transcriptions 3 () mRNA. In conclusion, ZEN enhances the biological effect of GH, promotes the development of the ovary (follicle), and exerts reproductive toxicity by increasing the expression level of , and mRNA ovary and immune intensity of GHR protein.
本实验旨在通过生长激素轴[生长激素释放激素（GHRH）/生长激素（GH）/生长激素受体（GHR）]来确定玉米赤霉烯酮（ZEN）对青春期前小母猪卵巢发育的影响。在一项为期40天的实验中，将48头长白×约克夏杂交的青春期前小母猪随机分配到四种日粮处理组，包括补充0（对照组）、400（T1）、800（T2）和1600（T3）μg/kg ZEN的基础日粮。与对照组相比，T2组（=0.058）和T3组（=0.065）的卵巢指数增加。此外，组织病理学检查显示，ZEN促进了卵巢和卵泡的发育。与对照组相比，T2实验组青春期前小母猪卵巢中的GHR含量、的相对表达水平、janus激活激酶2（）mRNA以及GHR的平均光密度在<0.05时显著增加。T3组的GHR含量、相对表达水平和转录信号转导子与激活子3（）mRNA显著更高。总之，ZEN增强了GH的生物学效应，促进了卵巢（卵泡）的发育，并通过增加卵巢中、和mRNA的表达水平以及GHR蛋白的免疫强度发挥生殖毒性作用。
