生长激素通过GHR-STAT3/5途径促进人子宫内膜腺细胞增殖和运动。
Growth hormone promotes human endometrial glandular cells proliferation and motion through the GHR-STAT3/5 pathway.
作者信息
Feng Qing, Gao Bingsi, Huang Huan, Zou Lingxiao, Zhao Xingping, Zhu Xiuting, Xu Dabao
机构信息
Department of Obstetrics and Gynecology, Third Xiangya Hospital of Central South University, Changsha 410013, China.
出版信息
Ann Transl Med. 2020 Feb;8(4):53. doi: 10.21037/atm.2019.12.08.
BACKGROUND
This study aims at investigating the effect of growth hormone (GH) on the growth of human endometrial glandular cells (hEGCs) and preliminary exploring its mechanism.
METHODS
HEGCs were isolated from the endometrial biopsies and exposed to different dose of GH (0, 50, 100, and 200 ng/mL). Cell proliferation and cell cycle assay, migration assay was performed to investigate the growth and motivation of hEGCs, respectively. Reverse transcription-polymerase chain reaction (RT-PCR), immunocytochemistry (ICC), and western blot (WB) were processed to investigate its related gene or protein expression.
RESULTS
The results revealed that GH administration promoted the proliferation, cell cycle, migration, and growth hormone receptors (GHRs) expression of the hEGC. We further inhibited GHRs with AG490, and the inhibitor reversed the effects of GH on cell growth, motion, and the activation of GHR and STAT3/5.
CONCLUSIONS
GH promoted hEGCs proliferation and motion, which is GHR-JAK-STAT3/5 signaling pathway-dependent. These findings reveal the essential roles of GH in the hEGCs growth and provide evidence for potential GH therapy in intrauterine adhesion (IUA) treatment.
背景
本研究旨在探讨生长激素（GH）对人子宫内膜腺细胞（hEGCs）生长的影响，并初步探索其机制。
方法
从子宫内膜活检组织中分离出hEGCs，并将其暴露于不同剂量的GH（0、50、100和200 ng/mL）。分别进行细胞增殖和细胞周期检测、迁移检测，以研究hEGCs的生长和运动情况。采用逆转录-聚合酶链反应（RT-PCR）、免疫细胞化学（ICC）和蛋白质印迹法（WB）检测其相关基因或蛋白表达。
结果
结果显示，给予GH可促进hEGC的增殖、细胞周期进程、迁移以及生长激素受体（GHRs）的表达。我们进一步用AG490抑制GHRs，该抑制剂逆转了GH对细胞生长、运动以及GHR和STAT3/5激活的影响。
结论
GH促进hEGCs的增殖和运动，这依赖于GHR-JAK-STAT3/5信号通路。这些发现揭示了GH在hEGCs生长中的重要作用，并为宫腔粘连（IUA）治疗中潜在的GH疗法提供了证据。
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