Song Tingting, Liu Xiufeng, Yuan Xuejun, Yang Weiren, Liu Faxiao, Hou Yanmeng, Huang Libo, Jiang Shuzhen
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an, China.
College of Life and Sciences, Shandong Agricultural University, Tai'an, China.
Front Vet Sci. 2021 Feb 4;8:629006. doi: 10.3389/fvets.2021.629006. eCollection 2021.
Zearalenone (ZEA) has an estrogen-like effect, which can injure the reproductive system of animals, causing infertility, and abortion in sows. However, the underlying mechanisms are still not clear. The objective of this study was to assess the effects of ZEA on the localization and expression of growth hormone (GH), growth hormone receptor (GHR), and heat shock protein 70 (Hsp70) in the ovaries of post-weaning gilts. Forty healthy post-weaning gilts were randomly provided one of four diets: normal basal diet supplemented with 0 (control), 0.5 (ZEA0.5), 1.0 (ZEA1.0), and 1.5 (ZEA1.5) mg ZEA/kg. Gilts were housed and fed individually for 35 days; the ovaries were collected after euthanasia for antioxidant index, relative mRNA and protein expression, and immunohistochemical analyses of GH, GHR, and Hsp70. The results revealed that the glutathione peroxidase and total superoxide dismutase levels decreased ( < 0.05), whereas the malondialdehyde level increased ( < 0.05) with increasing ZEA content. The localization pattern of GH, GHR, and Hsp70 in ZEA-treated gilts was the same as that in the control; however, the localization of yellow and brown immunoreactive substances of GH, GHR, and Hsp70 was stronger in the ZEA groups than in the control. The relative mRNA and protein expression of GHR and Hsp70 was the highest in the ZEA1.0 group ( < 0.05), whereas that of GH was the highest in the ZEA0.5 group ( < 0.05). The mRNA and protein expression of GH was lower in the ZEA1.5 group than in the control ( < 0.05). Hsp70 results showed adverse responses to increasing ZEA levels in gilt ovaries, suggesting that Hsp70 played an important role in alleviating ZEA-induced oxidative stress.
玉米赤霉烯酮（ZEA）具有雌激素样作用，可损害动物生殖系统，导致母猪不孕和流产。然而，其潜在机制仍不清楚。本研究的目的是评估ZEA对断奶后后备母猪卵巢中生长激素（GH）、生长激素受体（GHR）和热休克蛋白70（Hsp70）的定位和表达的影响。40头健康的断奶后后备母猪被随机提供四种日粮之一：添加0（对照）、0.5（ZEA0.5）、1.0（ZEA1.0）和1.5（ZEA1.5）mg ZEA/kg的正常基础日粮。母猪单独饲养并喂食35天；安乐死后收集卵巢进行抗氧化指标、相关mRNA和蛋白质表达以及GH、GHR和Hsp70的免疫组织化学分析。结果显示，随着ZEA含量增加，谷胱甘肽过氧化物酶和总超氧化物歧化酶水平降低（P<0.05），而丙二醛水平升高（P<0.05）。ZEA处理的后备母猪中GH、GHR和Hsp70的定位模式与对照相同；然而，ZEA组中GH、GHR和Hsp70的黄色和棕色免疫反应物质的定位比对照组更强。ZEA1.0组中GHR和Hsp70的相对mRNA和蛋白质表达最高（P<0.05），而ZEA0.5组中GH的相对mRNA和蛋白质表达最高（P<0.05）。ZEA1.5组中GH的mRNA和蛋白质表达低于对照组（P<0.05）。Hsp70结果表明后备母猪卵巢中对ZEA水平升高有不良反应，提示Hsp70在减轻ZEA诱导的氧化应激中起重要作用。
