鸡皮源胶原蛋白肽螯合锌通过抑制自噬促进锌吸收并抑制肿瘤生长和侵袭。

Chicken skin-derived collagen peptides chelated zinc promotes zinc absorption and represses tumor growth and invasion by suppressing autophagy.

作者信息

Liu Tengfei, Zou Lifang, Ji Xiaowen, Xiao Guiran

机构信息

China Light Industry Key Laboratory of Meat Microbial Control and Utilization, Hefei University of Technology, Hefei, China.

School of Food and Biological Engineering, Hefei University of Technology, Hefei, China.

出版信息

Front Nutr. 2022 Aug 3;9:960926. doi: 10.3389/fnut.2022.960926. eCollection 2022.

DOI:10.3389/fnut.2022.960926

PMID:35990359

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9381994/

Abstract

To improve the utilization value of chicken by-products, we utilized the method of step-by-step hydrolysis with bromelain and flavourzyme to prepare low molecular weight chicken skin collagen peptides (CCP) (<5 kDa) and characterized the amino acids composition of the CCP. Then, we prepared novel CCP-chelated zinc (CCP-Zn) by chelating the CCP with ZnSO. We found that the bioavailability of CCP-Zn is higher than ZnSO. Besides, CCP, ZnSO, or CCP-Zn effectively repressed the tumor growth, invasion, and migration in a malignant tumor model. Moreover, the anti-tumor activity of CCP-Zn is higher than CCP or ZnSO. Furthermore, the functional mechanism studies indicated that CCP, ZnSO, or CCP-Zn inhibits tumor progression by reducing the autonomous and non-autonomous autophagy in tumor cells and the microenvironment. Therefore, this research provides evidence for utilizing chicken skin in the development of zinc supplements and cancer treatment in the future.

摘要

为提高鸡副产品的利用价值，我们采用菠萝蛋白酶和风味酶分步水解的方法制备了低分子量鸡皮胶原蛋白肽（CCP，<5 kDa），并对CCP的氨基酸组成进行了表征。然后，通过将CCP与ZnSO螯合制备了新型CCP螯合锌（CCP-Zn）。我们发现CCP-Zn的生物利用度高于ZnSO。此外，CCP、ZnSO或CCP-Zn在恶性肿瘤模型中有效抑制了肿瘤的生长、侵袭和迁移。而且，CCP-Zn的抗肿瘤活性高于CCP或ZnSO。此外，功能机制研究表明，CCP、ZnSO或CCP-Zn通过减少肿瘤细胞和微环境中的自主和非自主自噬来抑制肿瘤进展。因此，本研究为未来利用鸡皮开发锌补充剂和癌症治疗提供了依据。