Hamed Abdallah Mohamed
Phys Deptartment, Fac. Sci., Ain Shams University, Cairo, 11566 Egypt.
Beni Suef Univ J Basic Appl Sci. 2022;11(1):98. doi: 10.1186/s43088-022-00276-2. Epub 2022 Aug 13.
The proposal of spiky apertures showed resolution improvement compared with the circular apertures. Three models of corona virus are given. The 1st model consists of uniform circular aperture provided with 8 spikes while the 2nd model has 16 spikes for the same uniform circular aperture. The 3rd model has circular linear distribution with 8 spikes.
The Normalized Point Spread Function (PSF) or the impulse response is computed for the three models using fast Fourier transform technique. In addition, the autocorrelation function corresponding to these apertures is calculated and compared with that corresponding to the ordinary circular and conic apertures. Coronavirus image is used as an object in the formation of images using confocal scanning laser microscope provided with suggested models. The fabricated MATLAB code is used to compute and plot all images and line plots.
The PSF plots are computed from Eqs. (8) and (12) using MATLAB code showing narrower cutoff in the PSF for spiky aperture compared with that corresponding to the uniform circular aperture and modulated linear and quadratic apertures. Hence, I reached resolution improvement in the case of spiky aperture.
与圆形孔径相比,尖刺孔径的提议显示出分辨率有所提高。给出了三种冠状病毒模型。第一种模型由带有8个尖刺的均匀圆形孔径组成,而第二种模型对于相同的均匀圆形孔径有16个尖刺。第三种模型具有带有8个尖刺的圆形线性分布。
使用快速傅里叶变换技术为这三种模型计算归一化点扩散函数(PSF)或脉冲响应。此外,计算与这些孔径对应的自相关函数,并将其与对应于普通圆形和圆锥孔径的自相关函数进行比较。在使用配备建议模型的共聚焦扫描激光显微镜形成图像时,将冠状病毒图像用作对象。所编写的MATLAB代码用于计算和绘制所有图像及线图。
使用MATLAB代码根据式(8)和(12)计算PSF图,结果表明与均匀圆形孔径以及调制线性和二次孔径相比,尖刺孔径的PSF截止更窄。因此,我得出在尖刺孔径情况下分辨率有所提高的结论。