Lindek S, Cremer C, Stelzer E H
Appl Opt. 1996 Jan 1;35(1):126-30. doi: 10.1364/AO.35.000126.
In a confocal theta fluorescence microscope, two objective lenses with circular apertures are used, one to illuminate the sample and the other to detect the emitted light at an angle to the illumination axis. We show that annular illumination and detection apertures lead to a reduction in the extent of the point-spread function. A spatial resolution improved by more than 50% can be achieved with a central obstruction blocking the inner 80% of the diameter. For the limit of a very narrow annular aperture and a numerical aperture of 0.75, the volume at half-maximum of the point-spread function is reduced from 15to5 aL. Amixed setup with anannular illumination aperture and a circular detection aperture is also considered.
在共焦θ荧光显微镜中，使用了两个带有圆形孔径的物镜，一个用于照亮样品，另一个用于在与照明轴成一定角度处检测发射光。我们表明，环形照明和检测孔径会导致点扩散函数范围的减小。通过中心遮挡阻挡直径的内80%，可以实现超过50%的空间分辨率提高。对于非常窄的环形孔径和数值孔径为0.75的极限情况，点扩散函数半高宽处的体积从15飞升减小到5飞升。还考虑了一种具有环形照明孔径和圆形检测孔径的混合设置。
