Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, USA.
Gastrovet, Sao Paulo, Brazil.
J Vet Diagn Invest. 2022 Nov;34(6):990-994. doi: 10.1177/10406387221119712. Epub 2022 Aug 22.
Granulomatous colitis in dogs can be associated with infection of the colonic mucosa by invasive strains of . To date, fluorescence in situ hybridization (FISH) is the gold-standard method to assess intramucosal and intracellular bacterial invasion. However, FISH requires expensive fluorescence microscopy equipment and is therefore not widely available. We investigated the use of immunohistochemistry (IHC) as an alternative method to detect invasive in dogs with granulomatous colitis. Archived paraffin-embedded blocks were selected from 26 dogs with colitis, in which FISH had been performed by an outside laboratory. Using a polyclonal antibody, IHC for was performed on sections cut from the same blocks, and the presence of invasive was recorded. All 11 specimens in which FISH had detected were also positive on IHC, with strong immunolabeling in the cytoplasm of macrophages and extracellularly in the lamina propria; all 15 specimens that were negative for invasive bacteria on FISH were also negative on IHC. We found that IHC is a sensitive technique for the detection of invasive in dogs with granulomatous colitis.
犬类肉芽肿性结肠炎可能与侵袭性结肠黏膜菌株感染有关。迄今为止，荧光原位杂交（FISH）是评估黏膜内和细胞内细菌侵袭的金标准方法。然而，FISH 需要昂贵的荧光显微镜设备，因此并不广泛适用。我们研究了免疫组织化学（IHC）作为替代方法，用于检测患有肉芽肿性结肠炎的犬类中的侵袭性。从外部实验室进行 FISH 的 26 只结肠炎犬的存档石蜡包埋块中选择了组织切片，使用多克隆抗体进行了针对 的 IHC 检测，并记录了侵袭性 的存在。在 FISH 检测到 的 11 个标本中，IHC 也呈阳性，巨噬细胞质和固有层细胞外有强烈的免疫标记；在 FISH 检测为阴性的 15 个标本中，IHC 也为阴性。我们发现，IHC 是一种用于检测犬类肉芽肿性结肠炎中侵袭性 的敏感技术。
