Laboratory of Environmental Remediation, College of Environmental and Chemical Engineering, Shanghai University, 99 Shangda Road, Shanghai, 200444, China.
Laboratory of Environmental Remediation, College of Environmental and Chemical Engineering, Shanghai University, 99 Shangda Road, Shanghai, 200444, China.
Environ Pollut. 2022 Oct 15;311:119970. doi: 10.1016/j.envpol.2022.119970. Epub 2022 Aug 19.
Microbial remediation is a potential remediation method for petroleum-contaminated soil. In order to explore the petroleum degradation mechanism by microorganisms, the oilfield soil was remedied by Acinetobacter baumannii combined with Talaromyces sp. The degradation mechanism was studied by analyzing soil microbial community and functional genes through metagenomics during the degradation process. The result showed the degradation rate of petroleum was 65.6% after 28 days. The concentration of petroleum decreased from 1220 mg/kg to 420 mg/kg. In the co-culture group, Acinetobacter baumannii became the dominant species, the annotated genes of it at the species level accounted for 7.34% while that of Talaromyces sp. accounted for only 0.34%. Meanwhile, the annotated genes of Bacillus, Halomonas, and Nitriliruptor at the genus level were up-regulated by 1.83%, 0.90%, and 0.71%, respectively. In addition, large functional genes were significantly up-regulated, including the peroxisome, P450 enzyme (CYP53, CYP116, CYP102, CYP645), and biofilm formulation, promoting the oxidation and hydroxylation, and catalyzing the epoxidation of aromatic and aliphatic hydrocarbons. Meanwhile, the degrading genes of alkanes and aromatic hydrocarbons were expressed promotionally, and degradation pathways were deduced. In conclusion, the inoculation of Acinetobacter baumannii combined with Talaromyces sp. accelerated the degradation of petroleum in oilfield soil and improved the growth of indigenous petroleum-degrading bacteria. Many functional genes related to petroleum degradation were promoted significantly. These results proved the co-culture of bacteria-fungi consortium contributes to the bioremediation of petroleum-contaminated soil.
微生物修复是一种有潜力的石油污染土壤修复方法。为了探索微生物对石油的降解机制，采用不动杆菌与拟青霉联合修复油田土壤，通过宏基因组分析微生物群落和功能基因，研究了石油在降解过程中的降解机制。结果表明，28 天后石油的降解率为 65.6%，石油浓度从 1220mg/kg 降至 420mg/kg。在共培养组中，不动杆菌成为优势种，其种水平注释基因占 7.34%，而拟青霉仅占 0.34%。同时，芽孢杆菌、盐单胞菌和硝化螺菌属的注释基因水平分别上调了 1.83%、0.90%和 0.71%。此外，大功能基因显著上调，包括过氧化物酶体、P450 酶（CYP53、CYP116、CYP102、CYP645）和生物膜形成，促进了芳烃和烷烃的氧化和羟化，以及环氧化反应。同时，促进了烷烃和芳烃降解基因的表达，并推断了降解途径。综上所述，接种不动杆菌与拟青霉联合可加速油田土壤中石油的降解，促进土著石油降解菌的生长。许多与石油降解相关的功能基因显著上调。这些结果证明了细菌-真菌共培养物有助于石油污染土壤的生物修复。
