College of Environmental and Chemical Engineering, Shanghai University, 99 Shangda Road, Shanghai, 200444, China.
College of Environmental and Chemical Engineering, Shanghai University, 99 Shangda Road, Shanghai, 200444, China.
Chemosphere. 2021 Jun;273:129666. doi: 10.1016/j.chemosphere.2021.129666. Epub 2021 Jan 16.
The purpose of this work was to study the biodegradation of crude oil under alkaline condition by defined co-culture of Acinetobacter baumannii and Talaromyces sp. The n-alkanes in crude oil could be completely degraded by bacteria and fungi with the ratio of 1:1 at pH 9 in 14 d water simulation experiment. Meanwhile, the total degradation rate of crude oil could reach 80%. Fungi had stronger ability to degrade n-alkanes, while bacteria could better degrade other components such as aromatics and branched alkanes. The two strains were both capable of producing a small amount of biosurfactant. High cell viability was the main factor for strains to exert high degradation ability in alkaline environment. It was preliminarily verified that bacteria and fungi rely on the differences of enzyme systems to achieve synergy in the degradation process. These results indicated that the defined co-culture had great potential for bioremediation in alkaline soils.
本工作旨在研究碱性条件下不动杆菌和塔宾曲霉的混合培养对原油的生物降解作用。在 14 d 的水模拟实验中，当 pH 值为 9 时，细菌和真菌的比例为 1:1 时，可完全降解原油中的直链烷烃。同时，原油的总降解率可达 80%。真菌具有更强的直链烷烃降解能力，而细菌则可以更好地降解芳烃和支链烷烃等其他成分。两株菌都能产生少量生物表面活性剂。高细胞活力是菌株在碱性环境中发挥高降解能力的主要因素。初步验证了细菌和真菌依靠酶系统的差异在降解过程中实现协同作用。这些结果表明，该明确的共培养物在碱性土壤的生物修复中具有巨大的潜力。
