长链非编码 RNA PCED1B-AS1 在肝癌中的上调及其对 miR-10a/BCL6 轴的调控促进细胞增殖。
LncRNA PCED1B-AS1 Upregulation in Hepatocellular Carcinoma and Regulation of the miR-10a/BCL6 Axis to Promote Cell Proliferation.
机构信息
Department of Clinical Laboratory, Inner Mongolia People's Hospital, Hohhot City, Inner Mongolia, 010021, PR China.
出版信息
Crit Rev Eukaryot Gene Expr. 2022;32(6):11-20. doi: 10.1615/CritRevEukaryotGeneExpr.2022039954.
Long noncoding RNA (lncRNA) PCED1B-AS1 has been characterized as an oncogene in glioma, but its role in hepatocellular carcinoma (HCC) is unknown. We explored the involvement of PCED1B-AS1 in HCC. Its expression in paired HCC and nontumor tissues from 62 HCC patients was determined by qRT-PCR. Correlations of PCED1B-AS1 with miR-10a and BCL6 were analyzed by Pearson's correlation coefficient. The patients were followed up for 5 years to analyze the prognostic value of PCED1B-AS1 for HCC. Interactions among PCED1B-AS1, miR-10a, and BCL6 were detected by overexpression experiments. Cell proliferation was analyzed by CCK-8 assay. We found the following. PCED1B-AS1 is upregulated in HCC, and its upregulation correlates with poor survival. Across HCC tissues, PCED1B-AS1 expression inversely correlates with miR-10a but positively correlates with BCL6. In HCC cells, overexpression of PCED1B-AS1 decreases miR-10a expression and increases BCL6 expression. Moreover, PCED1B-AS1 overexpression reduces the inhibitory effects of miR-10a overexpression on BCL6 expression and cell proliferation. PCED1B-AS1 is upregulated in HCC and regulates the miR-10a/BCL6 axis to promote cell proliferation.
长链非编码 RNA（lncRNA）PCED1B-AS1 已被鉴定为神经胶质瘤的癌基因，但在肝细胞癌（HCC）中的作用尚不清楚。我们探讨了 PCED1B-AS1 在 HCC 中的作用。通过 qRT-PCR 测定了 62 例 HCC 患者配对的 HCC 和非肿瘤组织中的 PCED1B-AS1 表达。通过 Pearson 相关系数分析了 PCED1B-AS1 与 miR-10a 和 BCL6 的相关性。对患者进行了 5 年的随访，以分析 PCED1B-AS1 对 HCC 的预后价值。通过过表达实验检测了 PCED1B-AS1、miR-10a 和 BCL6 之间的相互作用。通过 CCK-8 分析检测细胞增殖。我们发现以下结果：PCED1B-AS1 在 HCC 中上调，其上调与不良生存相关。在 HCC 组织中，PCED1B-AS1 表达与 miR-10a 呈负相关，与 BCL6 呈正相关。在 HCC 细胞中，过表达 PCED1B-AS1 可降低 miR-10a 的表达并增加 BCL6 的表达。此外，PCED1B-AS1 过表达可降低 miR-10a 过表达对 BCL6 表达和细胞增殖的抑制作用。PCED1B-AS1 在 HCC 中上调，并调节 miR-10a/BCL6 轴以促进细胞增殖。
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