Department of General Surgery, Affiliated Cixi Hospital, Wenzhou Medical University, 315300 Zhejiang Province, PR China.
Department of General Surgery, Affiliated Cixi Hospital, Wenzhou Medical University, 315300 Zhejiang Province, PR China.
Clin Res Hepatol Gastroenterol. 2021 Mar;45(2):101490. doi: 10.1016/j.clinre.2020.07.001. Epub 2021 Mar 18.
Long non-coding RNAs (LncRNAs) are broadly transcribed in the genome of human and animals, they play critical roles in cellular process, and participate in the progression of multiple diseases, including cancer. SLC16A1-AS1 is a tumor suppressive lncRNA in lung cancer. This study aimed to investigate the involvement of lncRNA SLC16A1-AS1 in hepatocellular carcinoma (HCC).
A total of 64 HCC patients were subjected to biopsy to obtain paired HCC and non-tumor tissues. Expression of SLC16A1-AS1 and miR-141 in paired tissues was determined by RT-qPCR. Correlations were analyzed by linear regression. Overexpression of SLC16A1-AS1 and miR-141 were achieved in HCC cells to explore the interactions between them. The methylation of the gene encoding miR-141 in HCC cells was detected by methylation-specific PCR (MSP). CCK-8 assay was performed for cell proliferation assay.
SLC16A1-AS1 was upregulated in HCC and its high expression levels predicted poor survival of HCC patients. Expression levels of miR-141 were lower in HCC patients and were inversely correlated with the expression levels of SLC16A1-AS1. In HCC cells, overexpression of SLC16A1-AS1 led to downregulation of miR-141, while overexpression of miR-141 did not regulate the expression of SLC16A1-AS1. In addition, overexpression of SLC16A1-AS1 led to increased methylation of miR-141. And overexpression of SLC16A1-AS1 attenuated the inhibitory effects of miR-141 on HCC cell proliferation.
SLC16A1-AS1 is upregulated in HCC and predicts poor survival. In addition, SLC16A1-AS1 may downregulate miR-141 through methylation to promote cancer cell proliferation.
长链非编码 RNA（lncRNA）广泛存在于人类和动物基因组中，它们在细胞过程中发挥着关键作用，并参与多种疾病的进展，包括癌症。SLC16A1-AS1 是肺癌中的一种肿瘤抑制性 lncRNA。本研究旨在探讨 lncRNA SLC16A1-AS1 在肝细胞癌（HCC）中的作用。
对 64 例 HCC 患者进行活检，获得配对的 HCC 和非肿瘤组织。采用 RT-qPCR 检测配对组织中 SLC16A1-AS1 和 miR-141 的表达。采用线性回归分析相关性。在 HCC 细胞中过表达 SLC16A1-AS1 和 miR-141，探讨它们之间的相互作用。采用甲基化特异性 PCR（MSP）检测 HCC 细胞中 miR-141 基因的甲基化。采用 CCK-8 法进行细胞增殖实验。
SLC16A1-AS1 在 HCC 中上调，其高表达水平预示着 HCC 患者的预后不良。HCC 患者 miR-141 的表达水平较低，与 SLC16A1-AS1 的表达水平呈负相关。在 HCC 细胞中，过表达 SLC16A1-AS1 导致 miR-141 下调，而过表达 miR-141 不能调节 SLC16A1-AS1 的表达。此外，过表达 SLC16A1-AS1 导致 miR-141 的甲基化增加。过表达 SLC16A1-AS1 减弱了 miR-141 对 HCC 细胞增殖的抑制作用。
SLC16A1-AS1 在 HCC 中上调，预示着预后不良。此外，SLC16A1-AS1 可能通过甲基化下调 miR-141 促进癌细胞增殖。
