Asghari Hanjani Nazanin, Zamaninour Negar, Athari Nik Azm Somayeh, Hosseini AghaFatemeh, Nasirinezhad Farinaz, Vafa Mohammad Reza
Department of Nutrition, School of Public Health, Iran University of Medical Sciences, Tehran, Iran.
Department of Minimally Invasive, Surgery Research Center, Iran University of Medical Sciences, Tehran, Iran.
Med J Islam Repub Iran. 2022 Feb 21;36:12. doi: 10.47176/mjiri.36.12. eCollection 2022.
Calorie Restriction (CR) is known as one of the most effective life-extending interventions. Therefore researchers are looking for other interventions or drugs to mimic the mentioned effects. Time-restricted feeding (TRF) has recently gained more attention recently as one of the CR mimetics. Here we evaluate and compare the effects of CR or TRF on cognitive function in young animals fed a high-fat diet (HFD). This is an experimental study that three-week-old male Wistar rats (n:52) were subjected to a control diet (n:11) or HFD (n:42). Then the HFD group was divided into 1) 30% calorie restriction (CR), 2) Night Intermittent Fasting (NIF), 3) Day Intermittent Fasting (DIF), and 4) Ad-Libitum (AL) with the standard diet for ten weeks (each of 9). An independent T-test or Mann-Whitney test was used for the first phase and in the second phase of the study, one-way analysis of variance (ANOVA), followed by Tukey post-hoc tests, or Kruskal-Wallis and post-hoc Bonferroni test were used. P-values of <0.05 were considered significant. Deteriorated mental function was significantly lower in HFD than CON (p= 0.041). CR was still more efficient than NIF in cognitive function in obese subjects. Post-hoc test indicated that from day 2-4, escape latency was significantly shorter in NIF and CR, which was not seen in other groups (p=0.045). While TRF has garnered much attention recently, here we show that CR is still more efficient in learning and memory tasks. Longer fasting times and different fasting periods are recommended to study.
热量限制（CR）是已知最有效的延长寿命的干预措施之一。因此，研究人员正在寻找其他干预措施或药物来模拟上述效果。限时进食（TRF）作为热量限制模拟物之一，最近受到了更多关注。在此，我们评估并比较了热量限制或限时进食对高脂饮食（HFD）喂养的幼龄动物认知功能的影响。这是一项实验研究，将3周龄雄性Wistar大鼠（n = 52）分为对照饮食组（n = 11）或高脂饮食组（n = 42）。然后将高脂饮食组分为1）30%热量限制（CR）、2）夜间间歇性禁食（NIF）、3）白天间歇性禁食（DIF）和4）自由进食（AL），给予标准饮食10周（每组9只）。研究的第一阶段使用独立样本t检验或曼-惠特尼检验，第二阶段使用单因素方差分析（ANOVA），随后进行Tukey事后检验，或使用Kruskal-Wallis检验和事后Bonferroni检验。P值<0.05被认为具有统计学意义。高脂饮食组的精神功能恶化程度显著低于对照组（p = 0.041）。在肥胖受试者中，热量限制在认知功能方面仍比夜间间歇性禁食更有效。事后检验表明，从第2 - 4天起，夜间间歇性禁食组和热量限制组的逃避潜伏期显著缩短，其他组未见此现象（p = 0.045）。虽然限时进食最近备受关注，但我们在此表明，热量限制在学习和记忆任务中仍然更有效。建议研究更长的禁食时间和不同的禁食时段。
