Saji S, Oshita H, Yamamoto S, Takekoshi T, Sakata K
J Surg Oncol. 1983 May;23(1):35-40. doi: 10.1002/jso.2930230110.
Immunologic merits and demerits of splenectomy were studied using a rat's experimental tumor. When splenectomy was done on day - 14, 2, or 14 of tumor inoculation, subsequent tumor growth was inhibited, but when it was done on day 7, tumor enhancement was observed. On day 2, cpm values of phytohemagglutinin (PHA)-induced blastogenesis of splenocytes was lower than in normal rats, on day 7 they significantly increased as compared with day 2, and on day 14 they significantly decreased as compared with day 7. Stimulation index (SI) ratio of PHA-induced blastogenesis and natural cell-mediated killing (NK) activity were also investigated using splenocytes, thymocytes, and peripheral lymphocytes. The results indicated that immunological competency of splenocytes of tumor bearers was reduced in the early, late, and final tumor-bearing periods, while it was increased in the middle tumor-bearing period. Tumor growth following splenectomy was considered to be controlled by these changes in immunological competency of splenocytes.
利用大鼠实验性肿瘤研究了脾切除术的免疫学优缺点。当在肿瘤接种后的第 - 14天、第2天或第14天进行脾切除时，随后的肿瘤生长受到抑制，但在第7天进行脾切除时，则观察到肿瘤增强。在第2天，脾细胞经植物血凝素（PHA）诱导的淋巴细胞转化的cpm值低于正常大鼠，在第7天，与第2天相比它们显著增加，而在第14天，与第7天相比它们显著降低。还使用脾细胞、胸腺细胞和外周淋巴细胞研究了PHA诱导的淋巴细胞转化的刺激指数（SI）比值和自然细胞介导的杀伤（NK）活性。结果表明，荷瘤大鼠脾细胞的免疫能力在荷瘤早期、晚期和末期降低，而在荷瘤中期增加。脾切除术后的肿瘤生长被认为受脾细胞免疫能力的这些变化控制。
