[Overexpression of 3 inhibits the differentiation of goat intramuscular preadipocytes].

The aim of this study was to investigate the effect of activating transcription factor 3 (3) on the differentiation of intramuscular preadipocytes in goat, and to elucidate its possible action pathway at the molecular level. In this study, the recombinant plasmid of goat pEGFP-N1-3 was constructed, and the intramuscular preadipocytes were transfected with liposomes. The relative expression levels of adipocyte differentiation marker genes were detected by quantitative real-time PCR (qRT-PCR). After transfection of goat intramuscular preadipocytes with the goat pEGFP-N1-3 overexpression vector, it was found that the accumulation of lipid droplets was inhibited, and the adipocyte differentiation markers , / and 1 were extremely significantly down-regulated ( < 0.01), while / and 2 were significantly down-regulated ( < 0.05). The 3 binding sites were predicted to exist in the promoter regions of , / and 2 by the ALGGEN PROMO program. The overexpression of goat 3 inhibits the accumulation of lipid droplets in intramuscular preadipocytes, and this effect may be achieved by down-regulating , / and 2. These results may facilitate elucidation of the regulatory mechanism of 3 in regulating the differentiation of goat intramuscular preadipocytes.