[miR-23b-3p regulates the differentiation of goat intramuscular preadipocytes by targeting the gene].

This study aimed to explore the effect of miR-23b-3p on the differentiation of goat intramuscular preadipocytes, and to confirm whether miR-23b-3p plays its roles targeting the gene. Based on the pre-transcriptome sequencing data obtained previously, the miR-23b-3p, which was differentially expressed in goat intramuscular adipocytes before and after differentiation, was used as an entry point. real-time quantitative-polymerase chain reaction (qPCR) was used to detect the expression pattern of miR-23b-3p during the differentiation of goat intramuscular preadipocytes. The effects of miR-23b-3p on adipose differentiation and adipose differentiation marker genes were determined at the morphological and molecular levels. The downstream target genes of miR-23b-3p were determined using bioinformatics prediction as well as dual luciferase reporter assay to clarify the targeting relationship between miR-23b-3p and the predicted target genes. The results indicated that overexpression of miR-23b-3p reduced lipid droplet accumulation in goat intramuscular adipocytes, significantly down-regulated the expression levels of adipogenic marker genes 2, /, , and ( < 0.01). In addition, the expressions of /, 2, 4 and were significantly downregulated ( < 0.05). After interfering with the expression of miR-23b-3p, lipid droplet accumulation was increased in goat intramuscular adipocytes. The expression levels of , , 2, 2, 4, and 1 were extremely significantly up-regulated ( < 0.01), and the expression levels of /, and were significantly up-regulated ( < 0.05). It was predicted that might be a target gene of miR-23b-3p. The mRNA expression level of was significantly decreased after overexpression of miR-23b-3p ( < 0.01), and the interference with miR-23b-3p significantly increased the mRNA level of ( < 0.05). The dual luciferase reporter assay indicated that miR-23b-3p had a targeting relationship with gene. MiR-23b-3p regulates the differentiation of goat intramuscular preadipocytes by targeting the gene.