Ghorbani Farnaz, Ghalandari Behafarid, Liu Zichen, Li Dejian, Yu Baoqing
Department of Orthopedics, Shanghai Pudong New Area People's Hospital, Shanghai, China.
Department of Orthopedics, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai, China.
Front Bioeng Biotechnol. 2022 Aug 8;10:967438. doi: 10.3389/fbioe.2022.967438. eCollection 2022.
This study aimed to develop injectable light-assisted thermo-responsive methylcellulose hydrogels filled with sodium humate, which were proposed for photothermal ablation and localized cisplatin delivery. Sodium humate converts light energy from laser beams into thermal energy, which causes methylcellulose to gel, thereby controlling the release of chemotherapy agents. Meanwhile, light emission causes to the photothermal ablation of tumor cells. For determining the optimal production conditions, different concentrations of sodium humate and light emission times were investigated. Results show that hydrogel uniformity is highly dependent on variables. An increase in sodium humate concentration and emission time resulted in a slight reduction in swelling ratio and an increase in durability. According to the simulation conditions, the cisplatin release profile was consistent with a non-Fickian mechanism with a predominant erosion contribution. In conjugation with increasing light emission time and sodium humate content, the storage modulus and viscosity increased, demonstrating hydrogel's sol-gel transition and long-lasting durability. The intrinsic fluorescence spectroscopy study revealed that the hydrogel-model protein complex empowered hydrogel bio-performance. Laser emission and cisplatin release synergistically reduced the number of viable osteosarcoma cell lines, suggesting the possibility of tumor ablation. This study describes the potential of simultaneous photothermal therapy and chemotherapy in osteosarcoma treatment, laying the groundwork for future preclinical and clinical trials.
本研究旨在开发填充有腐植酸钠的可注射光辅助热响应甲基纤维素水凝胶，该水凝胶被用于光热消融和局部顺铂递送。腐植酸钠将激光束的光能转化为热能，这会导致甲基纤维素凝胶化，从而控制化疗药物的释放。同时，发光会导致肿瘤细胞的光热消融。为了确定最佳生产条件，研究了不同浓度的腐植酸钠和发光时间。结果表明，水凝胶的均匀性高度依赖于变量。腐植酸钠浓度和发光时间的增加导致溶胀率略有降低，耐久性增加。根据模拟条件，顺铂释放曲线与以侵蚀为主的非菲克机制一致。随着发光时间和腐植酸钠含量的增加，储能模量和粘度增加，表明水凝胶的溶胶-凝胶转变和持久的耐久性。本征荧光光谱研究表明，水凝胶-模型蛋白复合物增强了水凝胶的生物性能。激光发射和顺铂释放协同减少了骨肉瘤细胞系的存活数量，表明肿瘤消融的可能性。本研究描述了光热疗法和化疗在骨肉瘤治疗中的潜在作用，为未来的临床前和临床试验奠定了基础。
