Kačániová Miroslava, Terentjeva Margarita, Kowalczewski Przemysław Łukasz, Babošová Mária, Porhajašová Jana Ivanič, Hikal Wafaa M, Fedoriak Mariia
Institute of Horticulture, Faculty of Horticulture and Landscape Engineering, Slovak University of Agriculture, Tr. A. Hlinku 2, 94976 Nitra, Slovakia.
Department of Bioenergy, Food Technology and Microbiology, Institute of Food Technology and Nutrition, University of Rzeszow, 4 Zelwerowicza St., 35-601 Rzeszow, Poland.
Insects. 2022 Jul 27;13(8):680. doi: 10.3390/insects13080680.
Arthropods are reported to serve as vectors of transmission of pathogenic microorganisms to humans, animals, and the environment. The aims of our study were (i) to identify the external bacteriota of spiders inhabiting a chicken farm and slaughterhouse and (ii) to detect antimicrobial resistance of the isolates. In total, 102 spiders of 14 species were collected from a chicken farm, slaughterhouse, and buildings located in west Slovakia in 2017. Samples were diluted in peptone buffered water, and Tryptone Soya Agar (TSA), Triple Sugar Agar (TSI), Blood Agar (BA), and Anaerobic Agar (AA) were used for inoculation. A total of 28 genera and 56 microbial species were isolated from the samples. The most abundant species were (28 isolates) and (28 isolates). The least isolated species were (one isolate), (two isolates), (two isolates), and (two isolates). There were differences in microbial composition between the samples originating from the slaughterhouse, chicken farm, and buildings. The majority of the bacterial isolates resistant to antibiotics were isolated from the chicken farm. The isolation of potentially pathogenic bacteria such as , , and spp., which possess multiple drug resistance, is of public health concern.
据报道，节肢动物是致病微生物向人类、动物和环境传播的媒介。我们研究的目的是：（i）鉴定栖息在养鸡场和屠宰场的蜘蛛体表微生物群，以及（ii）检测分离菌株的抗菌耐药性。2017年，从斯洛伐克西部的一个养鸡场、屠宰场及相关建筑中总共采集了14种102只蜘蛛。样本用蛋白胨缓冲液稀释后，接种于胰蛋白胨大豆琼脂（TSA）、三糖铁琼脂（TSI）、血琼脂（BA）和厌氧琼脂（AA）。从样本中总共分离出28个属和56种微生物。最常见的物种是（28株分离菌）和（28株分离菌）。最少见的分离物种是（1株分离菌）、（2株分离菌）、（2株分离菌）和（2株分离菌）。来自屠宰场、养鸡场和相关建筑的样本之间微生物组成存在差异。大多数对抗生素耐药的细菌分离株来自养鸡场。分离出具有多重耐药性的潜在致病细菌，如、和spp.，这引起了公共卫生方面的关注。
