Schött Hans-Frieder, Chua Esther W L, Mir Sartaj Ahmad, Burla Bo, Bendt Anne K, Wenk Markus R
Singapore Lipidomics Incubator, Life Sciences Institute, National University of Singapore, Singapore 117456, Singapore.
Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117596, Singapore.
Metabolites. 2022 Aug 5;12(8):723. doi: 10.3390/metabo12080723.
Quantitative analysis of bile acids in human feces can potentially help to better understand the influence of the gut microbiome and diet on human health. Feces is a highly heterogeneous sample matrix, mainly consisting of water and indigestible solid material (as plant fibers) that show high inter-individual variability. To compare bile acid concentrations among different individuals, a reliable normalization approach is needed. Here, we compared the impact of three normalization approaches, namely sample wet weight, dry weight, and protein concentration, on the absolute concentrations of fecal bile acids. Bile acid concentrations were determined in 70 feces samples from healthy humans. Our data show that bile acid concentrations normalized by the three different approaches are substantially different for each individual sample. Fecal bile acid concentrations normalized by wet weight show the narrowest distribution. Therefore, our analysis will provide the basis for the selection of a suitable normalization approach for the quantitative analysis of bile acids in feces.
对人类粪便中胆汁酸进行定量分析可能有助于更好地理解肠道微生物群和饮食对人类健康的影响。粪便作为一种高度异质的样本基质，主要由水和不可消化的固体物质（如植物纤维）组成，个体间差异很大。为了比较不同个体之间的胆汁酸浓度，需要一种可靠的标准化方法。在此，我们比较了三种标准化方法，即样本湿重、干重和蛋白质浓度，对粪便胆汁酸绝对浓度的影响。测定了70份健康人粪便样本中的胆汁酸浓度。我们的数据表明，对于每个个体样本，通过三种不同方法标准化的胆汁酸浓度存在显著差异。按湿重标准化的粪便胆汁酸浓度分布最窄。因此，我们的分析将为选择合适的标准化方法用于粪便中胆汁酸的定量分析提供依据。
