Nutrition and Food Science Area, Faculty of Pharmacy , University of Valencia , Avenida Vicente Andrés Estellés s/n , 46100 Burjassot , Valencia , Spain.
Laboratory of Lactic Acid Bacteria and Probiotics, Institute of Agrochemistry and Food Technology (IATA) . Spanish National Research Council (CSIC) , Avenida Agustín Escardino 7 , 46980 Paterna , Valencia , Spain.
J Agric Food Chem. 2020 Feb 19;68(7):1884-1895. doi: 10.1021/acs.jafc.9b04796. Epub 2019 Sep 26.
A beverage enriched with plant sterols (1 g/100 mL) and galactooligosaccharides (1.8 g/100 mL) was subjected to a dynamic gastrointestinal and colonic fermentation process to evaluate the effect on sterol metabolism, organic acid production, and microbiota composition. Production of sterol metabolites (coprostanol, methylcoprostanol, ethylcoprostenol, ethylcoprostanol, and sitostenone) was observed in the transverse colon (TC) and descending colon (DC) vessels in general, from 24 and 48 h, respectively. Microbial activity was assessed through the production of organic acids, mainly acetate in all colon vessels, lactate in the AC, and butyrate and propionate in the TC and DC. A higher diversity in the microbial community was found in the TC and DC, in accordance with a higher sterol metabolism and organic acid production. Although the prebiotic effect of galactooligosaccharides was not detected, changes in microbiota composition (an increase in the genus and the Synergistaceae and Lachnospiraceae families) indicated an enhancement of sterol metabolism.
一种富含植物甾醇（1 克/100 毫升）和半乳糖寡糖（1.8 克/100 毫升）的饮料经过动态胃肠道和结肠发酵过程，以评估其对甾醇代谢、有机酸生成和微生物组成的影响。甾醇代谢产物（粪甾醇、甲基粪甾醇、乙基粪甾醇、乙基粪甾醇和谷甾醇）通常在横结肠（TC）和降结肠（DC）血管中从 24 小时和 48 小时开始生成。微生物活性通过有机酸的产生来评估，所有结肠血管中的主要是乙酸，AC 中的是乳酸，TC 和 DC 中的是丁酸和丙酸。TC 和 DC 中的微生物群落多样性更高，与更高的甾醇代谢和有机酸生成一致。虽然没有检测到半乳糖寡糖的益生元作用，但微生物群落组成的变化（属和共生菌科和lachnospiraceae 科的增加）表明甾醇代谢增强。
