Gentile J M, Gentile G J, Plewa M J
Mutat Res. 1987 Jul;188(3):185-96. doi: 10.1016/0165-1218(87)90088-7.
We compared several phenylenediamines (4-nitro-o-phenylenediamine, NOP; 2-nitro-p-phenylenediamine, NPD; o-phenylenediamine, OPD; p-phenylenediamine, PPD; m-phenylenediamine, MPD) and aniline (ANL) for mutagenicity to Salmonella directly and following activation by plant and mammalian hepatic S9 using plate incorporation and preincubation protocols. In addition, we assayed each chemical for activation by intact plant cells using the plant cell/microbe coincubation protocol. At the concentrations tested, NOP, NPD, OPD, MPD and ANL were active in one or more assays. NPD, OPD and MPD were activated by mammalian hepatic S9 in one or more assay and each was activated by plant S9 or intact plant cells. ANL was mutagenic only in the presence of plant S9. PPD was not active under any of the test conditions.
我们使用平板掺入法和预孵育法，直接以及在植物和哺乳动物肝脏S9激活后，比较了几种苯二胺（4-硝基邻苯二胺，NOP；2-硝基对苯二胺，NPD；邻苯二胺，OPD；对苯二胺，PPD；间苯二胺，MPD）和苯胺（ANL）对沙门氏菌的致突变性。此外，我们使用植物细胞/微生物共孵育法检测了每种化学物质被完整植物细胞激活的情况。在所测试的浓度下，NOP、NPD、OPD、MPD和ANL在一种或多种检测中具有活性。NPD、OPD和MPD在一种或多种检测中被哺乳动物肝脏S9激活，并且每种都被植物S9或完整植物细胞激活。ANL仅在存在植物S9时具有致突变性。PPD在任何测试条件下均无活性。
