Gentile J M, Gentile G J, Plewa M J
Mutat Res. 1986 Feb;164(1):53-8. doi: 10.1016/0165-1161(86)90041-5.
We have studied the ability of two in vitro plant activation techniques to enhance the mutagenicity of 4-nitro-o-phenylenediamine (NOP) and to activate 2-aminofluorene (2AF). Mutagenic activities of NOP and 2AF were both increased by plant S9 in the Salmonella plate-incorporation and preincubation assays. They were also increased during preincubation with intact plant cells. NOP mutagenic activity was enhanced to a similar extent by plant S9 and by intact plant cells in Salmonella assay, whereas 2AF was activated more extensively by the plant cells than by plant S9. NOP was not enhanced by mammalian hepatic S9 in any assay, whereas 2AF was activated by hepatic S9 under all conditions tested.
我们研究了两种体外植物激活技术增强4-硝基邻苯二胺（NOP）致突变性以及激活2-氨基芴（2AF）的能力。在沙门氏菌平板掺入法和预孵育试验中，植物S9均增加了NOP和2AF的致突变活性。与完整植物细胞预孵育期间，它们的致突变活性也有所增加。在沙门氏菌试验中，植物S9和完整植物细胞对NOP致突变活性的增强程度相似，而植物细胞对2AF的激活作用比植物S9更广泛。在任何试验中，哺乳动物肝脏S9均未增强NOP的致突变活性，而在所有测试条件下，肝脏S9均可激活2AF。
