Plewa M J, Wagner E D, Gentile J M
Institute for Environmental Studies, University of Illinois, Urbana 61801.
Mutat Res. 1988 Feb;197(2):207-19. doi: 10.1016/0027-5107(88)90094-2.
The preincubation and suspension procedures of the plant cell/microbe coincubation assay are described and the activation of 2-aminofluorene and m-phenylenediamine by cultured tobacco, cotton, carrot and maize cells is compared. The assay measures the plant activation of promutagens into genotoxins detected in Salmonella typhimurium as well as toxicity in plant and microbial cells. At concentrations of 2-aminofluorene 0-0.5 mumoles/reaction tube, the rank order of the efficiency of activation by plant cells was tobacco much greater than cotton greater than carrot. Cultured maize cells did not activate 2-aminofluorene. The tobacco cell activation of 2-aminofluorene was inhibited 50% by 750 microM diethyldithiocarbamate under conditions that did not affect the cell viability. Tobacco cells were also the most efficient plant cells in activating m-phenylenediamine (0-5 mumoles/reaction tube). The 'biological affinity' of m-phenylenediamine for the activation system in tobacco cells was approximately 100 microM.
描述了植物细胞/微生物共培养试验的预孵育和悬浮程序，并比较了培养的烟草、棉花、胡萝卜和玉米细胞对2-氨基芴和间苯二胺的活化作用。该试验测定了植物将前诱变剂活化为在鼠伤寒沙门氏菌中检测到的基因毒素的能力，以及对植物和微生物细胞的毒性。在2-氨基芴浓度为0-0.5微摩尔/反应管时，植物细胞活化效率的排序为烟草远大于棉花大于胡萝卜。培养的玉米细胞不能活化2-氨基芴。在不影响细胞活力的条件下，750微摩尔二乙基二硫代氨基甲酸盐可抑制烟草细胞对2-氨基芴的活化作用50%。烟草细胞也是活化间苯二胺（0-5微摩尔/反应管）最有效的植物细胞。间苯二胺对烟草细胞活化系统的“生物亲和力”约为100微摩尔。
