Department of Aquatic Animals' Diseases and Management, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh, 13736, Egypt.
Department of Aquatic Animals' Diseases and Management, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh, 13736, Egypt.
Fish Shellfish Immunol. 2022 Sep;128:574-581. doi: 10.1016/j.fsi.2022.07.051. Epub 2022 Aug 22.
Nanotechnology has recently played a key role in tackling many aquacultures issues. Hence, the present study targets the evaluation of dietary inclusion of nano iron oxide (nFeO) on growth performance, hematology, immune-antioxidant responses, ionic regulation and expression of related genes in Nile tilapia (Oreochromis niloticus). Fish were fed supplementary nFeO at rates of zero (control), 0.5, and 1 g/kg diet for 30 days. Obtained data demonstrated that nFeO significantly (P < 0.05) augmented growth performance (final weight and length, body mass gain, specific growth rate, feed conversion ratio, and length gain rate). Hematological picture {RBCs, Hb, MCV, MCH and MCHC, and leukocytes interpretations (WBCs and monocytes)}; and biochemical indexes including (AST and ALT; total protein; and glucose, and cortisol) were significantly (P < 0.05) improved in nFeO supplemented groups. Plasma ionic concentration was also altered with nFeO supplementation, and 1g nFeO revealed the most marked increase in plasma (Na) potassium (K) levels. Similarly, IgM, nitrous oxide (NO), and lysozyme activity, plus superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities showed a remarkable improvement in 1g nFeO group compared to the control. Expression of Insulin-Like Growth Factor-1 (IGF-1) and interleukin 1-β (IL-1β) genes were significantly up-regulated in nFeO supplemented groups. Briefly, dietary nFeO inclusion had enhanced properties on growth; hemato-biochemical; immune, antioxidative profiles; and related genes expression of O. niloticus, with a recommended concentration of 1g nFeO.
纳米技术最近在解决许多水产养殖问题方面发挥了关键作用。因此，本研究旨在评估饲料中添加纳米氧化铁（nFeO）对尼罗罗非鱼（Oreochromis niloticus）生长性能、血液学、免疫抗氧化反应、离子调节和相关基因表达的影响。鱼用补充 nFeO 的饲料喂养，剂量分别为 0（对照）、0.5 和 1 g/kg 饲料，持续 30 天。结果表明，nFeO 显著（P < 0.05）提高了生长性能（终重和体长、体重增重、特定生长率、饲料转化率和体长增长率）。血液学指标{红细胞（RBCs）、血红蛋白（Hb）、平均红细胞体积（MCV）、平均红细胞血红蛋白量（MCH）和平均红细胞血红蛋白浓度（MCHC）、白细胞（WBCs 和单核细胞）}；和生化指标包括（AST 和 ALT；总蛋白；葡萄糖和皮质醇）在添加 nFeO 的组中显著（P < 0.05）提高。血浆离子浓度也随着 nFeO 的添加而改变，1 g nFeO 使血浆（Na）钾（K）水平显著增加。同样，1 g nFeO 组的免疫球蛋白 M（IgM）、亚硝酸盐（NO）和溶菌酶活性以及超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）活性与对照组相比也有显著提高。胰岛素样生长因子-1（IGF-1）和白细胞介素 1-β（IL-1β）基因的表达在添加 nFeO 的组中显著上调。总之，饲料中添加 nFeO 增强了 O. niloticus 的生长性能、血液生化、免疫抗氧化特性以及相关基因的表达，推荐添加浓度为 1 g nFeO。
