Hassanin Mohammed E, El-Murr Abdelhakeem, El-Khattib Amr R, Abdelwarith Abdelwahab A, Younis Elsayed M, Metwally Mohamed M M, Ismail Sameh H, Davies Simon J, Abdel Rahman Afaf N, Ibrahim Rowida E
Department of Aquatic Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, PO Box 44511, Zagazig, Sharkia, Egypt.
Department of Zoology, College of Science, King Saud University, PO Box 2455, Riyadh, 11451, Saudi Arabia.
Heliyon. 2024 Nov 14;10(22):e40418. doi: 10.1016/j.heliyon.2024.e40418. eCollection 2024 Nov 30.
The present investigation proposed an innovative trial at the probable beneficial effects of nano- (NBA) dietary incorporation on the Nile tilapia (). The investigation included the impact on the growth, digestive functions, immune-antioxidant indices, and resistance to challenge. A total of 135 fish (35.14 ± 0.12 g) were equally allocated into three groups (45 fish/group; 15 fish/replicate) in triplicates for 70 days. The control, NBA2, and NBA4 groups were fed basal diets enriched with 0, 10, and 10 CFU/kg NBA, respectively. Following the feeding experiment, all experimental groups were injected with 0.1 mL (1.5 × 10) and the fish mortalities were observed for 14 days. The outcomes showed that dietary NBA (NBA4 followed by the NBA2 diet) augmented the growth variables (final body weight, total weight gain, and specific growth rate) and condition factor and declined the feed conversion ratio. The intestinal digestive enzyme (amylase and lipase) and growth hormone levels were increased and the serum glucose level was decreased by dietary NBA. Furthermore, NBA diets enhanced the immune (total protein, globulin, lysozyme, complement 3, myeloperoxidase, and phagocytic activity) and antioxidant (superoxide dismutase, catalase, and total antioxidant capacity) parameters. The intestinal histology revealed no pathological lesions with a significant improvement in the intestinal histomorpho-measures (villus height and width, villus surface area, lamina propria thickness, and tunica muscularis thickness) by NBA diets in a dose-dependent manner. In the fish intestine, the count was increased in the NBA groups (NBA4 group followed by NBA2 group) with no discernible difference in the total bacterial count. Fish resistance to the challenge was increased by increasing the survival % in the NBA4 group (91.70 %) followed by the NBA2 group (83.30 %) compared to the control group (70.80 %). Overall, dietary NBA (especially 10 CFU/kg diet) could be a promising feed supplement in the Nile tilapia diets for improving their growth, health, and resistance to bacterial challenges.
本研究针对纳米生物活性添加剂（NBA）添加到尼罗罗非鱼日粮中可能产生的有益效果开展了一项创新性试验。该研究涵盖了对尼罗罗非鱼生长、消化功能、免疫抗氧化指标以及抗嗜水气单胞菌攻击能力的影响。总共135尾鱼（35.14 ± 0.12克）被平均分为三组（每组45尾鱼；每重复15尾鱼），进行三次重复，为期70天。对照组、NBA2组和NBA4组分别投喂添加了0、10和10⁶CFU/千克NBA的基础日粮。投喂实验结束后，所有实验组均注射0.1毫升（1.5×10⁸）嗜水气单胞菌，并观察14天的鱼类死亡率。结果表明，日粮中添加NBA（NBA4组其次是NBA2组）提高了生长变量（终末体重、总增重和特定生长率）和肥满度，降低了饲料转化率。日粮中添加NBA可提高肠道消化酶（淀粉酶和脂肪酶）和生长激素水平，并降低血清葡萄糖水平。此外，NBA日粮增强了免疫（总蛋白、球蛋白、溶菌酶、补体3、髓过氧化物酶和吞噬活性）和抗氧化（超氧化物歧化酶、过氧化氢酶和总抗氧化能力）参数。肠道组织学检查显示无病理损伤，且NBA日粮以剂量依赖的方式显著改善了肠道组织形态学指标（绒毛高度和宽度、绒毛表面积、固有层厚度和肌层厚度）。在鱼肠道中，NBA组（NBA4组其次是NBA2组）的嗜水气单胞菌数量增加，而总细菌数量无明显差异。与对照组（70.80%）相比，NBA4组（91.70%）其次是NBA2组（83.30%）的鱼类存活率提高，增强了鱼对嗜水气单胞菌攻击的抵抗力。总体而言，日粮中添加NBA（尤其是10⁶CFU/千克日粮）可能是尼罗罗非鱼日粮中一种有前景的饲料添加剂，可改善其生长、健康状况和对细菌攻击的抵抗力。
