Laboratory for Stem Cell & Regenerative Medicine, Natural and Medical Sciences Research Center, University of Nizwa, Nizwa, Oman.
Natural products Laboratory, Natural and Medical Sciences Research Center, University of Nizwa, Nizwa, Oman.
Nat Prod Res. 2023 Jun;37(12):2049-2054. doi: 10.1080/14786419.2022.2116024. Epub 2022 Aug 25.
This study aimed to develop a local 3 D-printed bioactive graft using poly-caprolacton (PCL) as a drug carrier and 3--acetyl-β-boswellic acid (β-ABA) as an anticancer compound. β-ABA-loaded 3 D-printed scaffold was fabricated and physically characterized. The results indicated more desirable mechanical and physical properties of the β-ABA-loaded PCL mat in comparison with the PCL scaffold. Following sustained release of β-ABA, the β-ABA-loaded PCL scaffold revealed selective cytotoxic activity against melanoma cells, while the PCL + ABA with the bolus delivery of β-ABA was toxic against fibroblast cells. Followed by the induction of apoptosis in melanoma cells at the gene level, the result of the western blot showed that the β-ABA-loaded scaffold significantly up-regulated P53 and down-regulated BCL2, with an increment in the ratio of Bax/BCL2. The selective anti-cancer properties of β-ABA-loaded 3 D printed scaffold against melanoma cells indicated that this scaffold could be potentially used as a bioactive graft to improve the melanoma treatment.
本研究旨在开发一种局部 3D 打印的生物活性移植物，使用聚己内酯（PCL）作为药物载体和 3--乙酰基-β-乳香酸（β-ABA）作为抗癌化合物。负载β-ABA 的 3D 打印支架被制备并进行了物理特性表征。结果表明，与 PCL 支架相比，负载β-ABA 的 PCL 基质具有更理想的机械和物理性能。β-ABA 的持续释放后，负载β-ABA 的 PCL 支架对黑色素瘤细胞表现出选择性细胞毒性，而 PCL+ABA 中β-ABA 的突释则对成纤维细胞有毒性。在基因水平诱导黑色素瘤细胞凋亡后，western blot 的结果表明，负载β-ABA 的支架显著上调了 P53 并下调了 BCL2，Bax/BCL2 的比值增加。负载β-ABA 的 3D 打印支架对黑色素瘤细胞的选择性抗癌特性表明，该支架可潜在用作生物活性移植物，以改善黑色素瘤的治疗效果。
