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人血小板裂解液作为培养营养添加剂对人乳牙牙髓干细胞体外扩增的影响。

Effect of Human Platelet Lysate as Cultivation Nutrient Supplement on Human Natal Dental Pulp Stem Cell In Vitro Expansion.

机构信息

Department of Dentistry, Charles University, Faculty of Medicine in Hradec Kralove, University Hospital Hradec Kralove, 500 05 Hradec Kralove, Czech Republic.

Department of Histology and Embryology, Charles University, Faculty of Medicine in Hradec Kralove, 500 03 Hradec Kralove, Czech Republic.

出版信息

Biomolecules. 2022 Aug 8;12(8):1091. doi: 10.3390/biom12081091.

DOI:10.3390/biom12081091
PMID:36008985
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9405745/
Abstract

Despite several scientific or ethical issues, fetal bovine serum (FBS) remains the standard nutrient supplement in the mesenchymal stem cell cultivation medium. Cell amplification plays an important role in human stem cell therapies. Increasing interest in this field has supported attempts to find suitable human alternatives to FBS for in vitro cell propagation. Human platelet lysate (hPL) has recently been determined as one of them. Our study aimed to evaluate the influence of 2% hPL in the growth medium for in vitro expansion of human natal dental pulp stem cells (hNDP-SCs). The effect was determined on proliferation rate, viability, phenotype profile, expression of several markers, relative telomere length change, and differentiation potential of four lineages of hNDP-SCs. As a control, hNDP-SCs were simultaneously cultivated in 2% FBS. hNDP-SCs cultivated in hPL showed a statistically significantly higher proliferation rate in initial passages. We did not observe a statistically significant effect on mesenchymal stem cell marker (CD29, CD44, CD73, CD90) or stromal-associated marker (CD13, CD166) expression. The cell viability, relative telomere length, or multipotency remained unaffected in hNDP-SCs cultivated in hPL-medium. In conclusion, hPL produced under controlled and standardized conditions is an efficient serum supplement for in vitro expansion of hNDP-SCs.

摘要

尽管存在一些科学或伦理问题，但胎牛血清（FBS）仍然是间充质干细胞培养中的标准营养补充剂。细胞扩增在人类干细胞治疗中起着重要作用。人们对该领域越来越感兴趣，这支持了寻找合适的人类替代品来替代 FBS 进行体外细胞繁殖的尝试。人血小板裂解液（hPL）最近被确定为其中之一。我们的研究旨在评估 2% hPL 在生长培养基中对人天然牙髓干细胞（hNDP-SCs）体外扩增的影响。通过增殖率、活力、表型谱、几种标志物的表达、相对端粒长度变化以及 hNDP-SCs 的四个谱系的分化潜能来确定其效果。作为对照，hNDP-SCs 同时在 2% FBS 中培养。在 hPL 中培养的 hNDP-SCs 在初始传代中表现出统计学上显著更高的增殖率。我们没有观察到对间充质干细胞标志物（CD29、CD44、CD73、CD90）或基质相关标志物（CD13、CD166）表达的统计学显著影响。在 hPL 培养基中培养的 hNDP-SCs 的细胞活力、相对端粒长度或多能性不受影响。总之，在受控和标准化条件下生产的 hPL 是 hNDP-SCs 体外扩增的有效血清补充剂。

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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1bb/9405745/a383cfb29b07/biomolecules-12-01091-g010a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1bb/9405745/1a8604365103/biomolecules-12-01091-g011.jpg
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