Centro Universitario di Odontoiatria, Università di Parma, Via Gramsci 14, 43126 Parma, Italy.
Dipartimento di Medicina e Chirurgia, Università di Parma, Via Gramsci 14, 43126 Parma, Italy.
Biomolecules. 2022 Aug 21;12(8):1159. doi: 10.3390/biom12081159.
The aim of this study was to investigate the effects of the androgenic hormone testosterone enanthate (TE) on human MG-63 cells. MG-63 were cultured for 24 h in the presence of TE at increasing concentrations to assess its lethal dose. Therefore, the suitable concentration for a prolonged use of TE in vitro was assessed by viability assay over 9 days. Finally, MG-63 were exposed to TE for 14 days and assayed for differentiation by qPCR and Alizarin Red S staining. TE in the amount of 100 µM resulted as the maximum dose tolerated by MG-63 cells after 24 h. However, a prolonged exposure in culture TE in the amount of 100 µM showed a cytostatic effect on cell proliferation. On the contrary, TE 10 µM was tolerated by the cells and did not boost cell proliferation, but did enhance new bone formation, as revealed by , , , and gene expression after 3, 7, and 14 days, and calcium deposition by Alizarin Red S staining after 14 days. Based on the current study, 10 µM is the critical dose of TE that should be used in vitro to support bone differentiation of MG-63 cells.
本研究旨在探讨雄激素睾酮庚酸酯（TE）对人 MG-63 细胞的影响。将 MG-63 在含有 TE 的培养基中培养 24 小时，以评估其致死剂量。因此，通过 9 天的细胞活力测定评估了 TE 体外长期使用的合适浓度。最后，将 MG-63 暴露于 TE 中 14 天，并通过 qPCR 和茜素红 S 染色检测分化。100µM 的 TE 是 MG-63 细胞在 24 小时后耐受的最大剂量。然而，在培养中延长暴露于 100µM 的 TE 对细胞增殖显示出细胞抑制作用。相反，10µM 的 TE 被细胞耐受，不会促进细胞增殖，但会增强新骨形成，这在第 3、7 和 14 天时通过 、 、 和 基因表达以及第 14 天时茜素红 S 染色的钙沉积得到证实。基于本研究，10µM 是 TE 的临界剂量，应在体外用于支持 MG-63 细胞的骨分化。
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