Song Xindi, Liu Junfeng, Wang Yanan, Zheng Lukai, Liu Ming
Center of Cerebrovascular Diseases, Department of Neurology, West China Hospital of Sichuan University, Chengdu 610041, China.
Institute for Stroke and Dementia Research, Ludwig Maximilian University Hospital of Munich (KUM), 81377 Munich, Germany.
Brain Sci. 2022 Jul 28;12(8):999. doi: 10.3390/brainsci12080999.
It is unclear whether miR-491-5p, miR-206, miR-21-5p or miR-3123 are associated with functional outcomes and hemorrhagic transformation (HT) after acute ischemic stroke (AIS). In this study, we aimed to investigate the correlation between these four microRNAs and functional outcomes, as well as spontaneous HT after AIS; Methods: We included 215 AIS patients and retrospectively assayed for miR-21-5p, miR-206, miR-3123 and miR-491-5p levels in serum. Poor functional outcome was defined as a modified Rankin Scale score ≥ 3. Spontaneous HT referred to hemorrhage detected in follow-up brain imaging but not on admission, without reperfusion therapies. Logistic regression, generalized additive model and 2-piecewise regression model were used to explore the independent, non-linear correlation between miRNA expression levels and outcomes; Results: We included 215 AIS patients. Higher miR-491-5p level independently reduced the risk of poor functional outcomes at 1 year (OR 0.90, 95% CI 0.82-0.98, corrected value = 0.044). Higher miR-206 level significantly increased the risk of spontaneous HT (OR 1.64, 95% CI 1.17-2.30, corrected value = 0.016). There was a nonlinear correlation found between miR-491-5p level and 1 year outcome with an inflection point of 2.180, while an approximately linear correlation was observed with an inflection point of 2.037 between miR-206 level and spontaneous HT; Conclusions: Higher serum miR-491-5p level independently reduced risk of 1-year poor functional outcome of AIS patients. Higher serum miR-206 level independently increased the risk of spontaneous HT in AIS patients. These two miRNAs may be as the potential biomarkers for improving prognosis after AIS.
目前尚不清楚miR-491-5p、miR-206、miR-21-5p或miR-3123是否与急性缺血性卒中（AIS）后的功能结局和出血性转化（HT）相关。在本研究中，我们旨在探讨这四种 microRNA 与功能结局以及 AIS 后自发性 HT 之间的相关性；方法：我们纳入了215例AIS患者，并回顾性检测了血清中miR-21-5p、miR-206、miR-3123和miR-491-5p的水平。功能结局差定义为改良Rankin量表评分≥3分。自发性HT是指在随访脑成像中检测到出血，但入院时未检测到，且未进行再灌注治疗。采用逻辑回归、广义相加模型和两段式回归模型探讨miRNA表达水平与结局之间的独立、非线性相关性；结果：我们纳入了215例AIS患者。较高的miR-491-5p水平独立降低了1年时功能结局差的风险（OR 0.90，95%CI 0.82-0.98，校正P值 = 0.044）。较高的miR-206水平显著增加了自发性HT的风险（OR 1.64，95%CI 1.17-2.30，校正P值 = 0.016）。发现miR-491-5p水平与1年结局之间存在非线性相关性，拐点为2.180，而miR-206水平与自发性HT之间在拐点为2.037时观察到近似线性相关性；结论：较高的血清miR-491-5p水平独立降低了AIS患者1年时功能结局差的风险。较高的血清miR-206水平独立增加了AIS患者自发性HT的风险。这两种miRNA可能作为改善AIS后预后的潜在生物标志物。
