Martínez-Zamora Lorena, Castillejo Noelia, Artés-Hernández Francisco
Postharvest and Refrigeration Group, Department of Agronomical Engineering & Institute of Plant Biotechnology, Universidad Politécnica de Cartagena, 30203 Cartagena, Spain.
Department of Food Technology, Nutrition, and Food Science, Faculty of Veterinary Sciences, University of Murcia, Espinardo, 30071 Murcia, Spain.
Antioxidants (Basel). 2022 Jul 29;11(8):1490. doi: 10.3390/antiox11081490.
Ultrasounds (US) and LED illumination are being studied to optimize yield and quality. The objective was to evaluate the effect of a pre-sowing US treatment combined with a postharvest photoperiod including LEDs on rocket sprouts’ quality and phytochemicals during shelf life. A US treatment (35 kHz; 30 min) applied to seeds and a postharvest photoperiod of 14 h fluorescent light (FL) + 10 h White (W), Blue (B), Red (R) LEDs or Darkness (D) were assayed. Antioxidants as phenolics and sulfur compounds (glucosinolates and isothiocyanates) were periodically monitored over 14 days at 5 °C. The US treatment increased the sulforaphane content by ~4-fold compared to CTRL seeds and sprouts. The phenolic acids and the flavonoid biosynthesis were enhanced by ~25%, ~30%, and ~55% under photoperiods with W, B, and R, respectively, compared to darkness. The total glucosinolate content was increased by >25% (W) and >45% (B and R) compared to darkness, which also reported increases of ~2.7-fold (W), ~3.6-fold (B), and ~8-fold (R) of the sulforaphane content as a main isothiocyanate. Postharvest lighting is an interesting tool to stimulate the secondary metabolism, while a US treatment was able to increase the sulforaphane content in seeds and sprouts, although no synergistic effect was reported.
目前正在研究超声波（US）和LED照明以优化产量和品质。目的是评估播种前超声处理与包括LED在内的收获后光周期对芝麻菜芽苗菜货架期品质和植物化学物质的影响。对种子进行了US处理（35千赫；30分钟），并测定了收获后的光周期，即14小时荧光灯（FL）+10小时白色（W）、蓝色（B）、红色（R）LED灯或黑暗（D）处理。在5℃下，每隔14天定期监测酚类和硫化合物（硫代葡萄糖苷和异硫氰酸盐）等抗氧化剂。与对照种子和芽苗菜相比，US处理使萝卜硫素含量增加了约4倍。与黑暗处理相比，在W、B和R光周期下，酚酸和类黄酮生物合成分别提高了约25%、30%和55%。与黑暗处理相比，总硫代葡萄糖苷含量增加了>25%（W）和>45%（B和R），作为主要异硫氰酸盐的萝卜硫素含量也分别增加了约2.7倍（W）、3.6倍（B）和8倍（R）。收获后照明是刺激次生代谢的一种有趣工具，而US处理能够增加种子和芽苗菜中的萝卜硫素含量，尽管未报道有协同效应。
