接受补充饲料的尼罗罗非鱼的生理性能、抗氧化和免疫状态、柱状病抗性及生长情况。

Physiological Performance, Antioxidant and Immune Status, Columnaris Resistance, and Growth of Nile Tilapia That Received -Supplemented Diets.

作者信息

Mansour Abdallah Tageldein, Mahboub Heba H, Elshopakey Gehad E, Aziz Enas K, Alhajji Adnan H M, Rayan Gamal, Ghazzawy Hesham S, El-Houseiny Walaa

机构信息

Aquaculture and Animal Production Department, Agricultural & Food Sciences College, King Faisal University, P.O. Box 400, Al-Ahsa 31982, Saudi Arabia.

Fish and Animal Production Department, Faculty of Agriculture (Saba Basha), Alexandria University, Alexandria 21531, Alexandria, Egypt.

出版信息

Antioxidants (Basel). 2022 Jul 29;11(8):1494. doi: 10.3390/antiox11081494.

DOI:10.3390/antiox11081494

PMID:36009213

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9404728/

Abstract

The current perspective is a pioneering trial to assess the efficacy of the dietary supplementation of powder (AVP) in the diet of Nile tilapia () on growth performance, blood picture, hepatic and renal biomarkers, immune status, and serum and tissue antioxidant capacity and to investigate the resistance against challenge. Fish ( = 360) were distributed into six groups (three replicates each) and received increasing AVP supplementation levels (0, 2, 4, 6, 8, and 10 g kg) for 60 days. Furthermore, fish were exposed to the bacterial challenge of a virulent strain and maintained under observation for 12 days. During the observation period, clinical signs and the cumulative mortality percentage were recorded. The results demonstrated that the growth performance, feed conversion ratio, and hematological profile were noticeably enhanced in the AVP-supplemented groups compared to the control. The most promising results of weight gain and feed conversion ratio were recorded in the groups with 6, 8, and 10 g AVP kg diets in a linear regression trend. The levels of hepatorenal function indicators were maintained in a healthy range in the different dietary AVP-supplemented groups. In a dose-dependent manner, fish fed AVP dietary supplements displayed significant augmented serum levels of innate immune indicators (lysozyme, nitric oxide, and complement 3) and antioxidant biomarkers (Catalase (CAT), superoxide dismutase (SOD), total antioxidant (TAC), and reduced glutathione (GSH) with a marked decrease in myeloperoxidase (MPO) and malondialdehyde (MDA) levels). Likewise, hepatic CAT and SOD activities were significantly improved, and the opposite trend was recorded with hepatic MDA. The highest AVP-supplemented dose (10 g/kg) recorded the highest immune-antioxidant status. Based on the study findings, we highlight the efficacy of AVP as a nutraceutical dietary supplementation for aquaculture to enhance growth, physiological performance, and immune-antioxidant status and as a natural economic antibacterial agent in for sustaining aquaculture. It could be concluded that the dietary supplementation of 10 g AVP/kg enhanced growth, physiological performance, immune-antioxidant status, and resistance against

摘要

当前的研究是一项开创性试验，旨在评估在尼罗罗非鱼（Oreochromis niloticus）日粮中添加粉末状的AVP对其生长性能、血液指标、肝脏和肾脏生物标志物、免疫状态以及血清和组织抗氧化能力的影响，并研究其对嗜水气单胞菌（Aeromonas hydrophila）攻击的抵抗力。将360尾鱼分为六组（每组三个重复），并在60天内给予递增水平的AVP补充剂（0、2、4、6、8和10 g/kg）。此外，使鱼暴露于强毒嗜水气单胞菌菌株的细菌攻击，并观察12天。在观察期内，记录临床症状和累积死亡率百分比。结果表明，与对照组相比，添加AVP的组的生长性能、饲料转化率和血液学指标显著提高。在日粮中添加6、8和10 g AVP/kg的组中，增重和饲料转化率的结果最有前景，呈线性回归趋势。在不同日粮添加AVP的组中，肝肾功指标水平维持在健康范围内。以剂量依赖的方式，喂食AVP日粮补充剂的鱼血清中先天免疫指标（溶菌酶、一氧化氮和补体3）和抗氧化生物标志物（过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、总抗氧化剂（TAC）和还原型谷胱甘肽（GSH））的水平显著升高，而髓过氧化物酶（MPO）和丙二醛（MDA）水平显著降低。同样，肝脏CAT和SOD活性显著提高，而肝脏MDA则呈现相反趋势。添加最高剂量AVP（10 g/kg）的组免疫抗氧化状态最高。基于研究结果，我们强调AVP作为水产养殖的营养膳食补充剂，可提高生长、生理性能和免疫抗氧化状态，以及作为嗜水气单胞菌中的天然经济抗菌剂以维持水产养殖的功效。可以得出结论，日粮中添加10 g AVP/kg可提高尼罗罗非鱼的生长、生理性能、免疫抗氧化状态以及对嗜水气单胞菌的抵抗力。