Tamagno Wagner Antonio, Alves Carla, Tessaro Diego, Sutorillo Nathália Tafarel, Santin Wallace, Barcellos Leonardo José Gil
Biochemistry Profª Drª Rosilene Rodrigues Kaizer Laboratory of the Federal Institute of Education, Science, and Technology of Rio Grande do Sul, Sertão Campus, Sertão 99170-000, RS, Brazil.
Graduate Program in Pharmacology, Universidade Federal de Santa Maria (UFSM), Santa Maria 97105-900, RS, Brazil.
Antioxidants (Basel). 2022 Jul 31;11(8):1507. doi: 10.3390/antiox11081507.
(Herb mate) is a native plant from South America, widely consumed through the infusion of dried leaves. The presence of antioxidant properties in herb mate may be relevant and contribute to evaluating the effect of its compounds against oxidative stress, which could cause neurodegenerative diseases. Despite having health benefits, there are reports of the presence of heavy metals in extracts obtained from the infusion. One of these metals is iron (Fe), found in large amounts in herb mate. To reverse the cumulative effects of metals and Fe in the body, the use of Deferoxamine (Dfx) is indicated, being a potent chelator of Fe. In this work, we aimed to evaluate the antioxidant potential of the micro-encapsulated extract of (MEIP) supplemented with Dfx on zebrafish behavior and biochemical biomarkers. To evaluate the effect and the supplementation, four groups were established: the first group was the control (water); the second, fish treated with MEIP; the third group was formed of fish treated with Dfx; while the fourth group was treated with both MEIP and Dfx. When applied alone, Dfx presents an anxiogenic-like pattern on zebrafish (), while the MEIP shows an anxiolytic-like behavior. The antioxidant enzymes are re-modulated close to control when the MEIP + Dfx is applied. The cholinergic system shows an activation of the signaling, as well as the heme radical group formation, which is not affected by the Dfx-chelating effect. Thus, the supplementation of MEIP with Dfx is important to transform this extract into one that is safer and healthier for human consumption.
马黛茶是一种原产于南美洲的植物，人们广泛饮用其干叶泡制的饮品。马黛茶中存在的抗氧化特性可能具有重要意义，并有助于评估其化合物对氧化应激的影响，氧化应激可能导致神经退行性疾病。尽管马黛茶有益健康，但有报道称其泡制提取物中存在重金属。其中一种金属是铁（Fe），在马黛茶中含量很高。为了逆转体内金属和铁的累积效应，建议使用去铁胺（Dfx），它是一种有效的铁螯合剂。在这项研究中，我们旨在评估添加Dfx的微囊化马黛茶提取物（MEIP）对斑马鱼行为和生化生物标志物的抗氧化潜力。为了评估MEIP和添加物的效果，设立了四组：第一组为对照组（水）；第二组，用MEIP处理的鱼；第三组由用Dfx处理的鱼组成；而第四组同时用MEIP和Dfx处理。单独使用时，Dfx在斑马鱼身上呈现出类似焦虑的模式（），而MEIP则表现出类似抗焦虑的行为。当应用MEIP + Dfx时，抗氧化酶的调节接近对照组。胆碱能系统显示出信号激活以及血红素自由基基团的形成，这不受Dfx螯合作用的影响。因此，用Dfx补充MEIP对于将这种提取物转化为对人类消费更安全、更健康的产品很重要。
