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不定根和毛状根培养作为木脂素植物工厂

Adventitious and Hairy-Roots Cultures as Lignan Plant Factories.

作者信息

Dougué Kentsop Roméo Arago, Consonni Roberto, Alfieri Michela, Laura Marina, Ottolina Gianluca, Mascheretti Iride, Mattana Monica

机构信息

Institute of Agricultural Biology and Biotechnology, National Research Council, Via Bassini 15, 20133 Milan, Italy.

Institute of Chemical Sciences and Technologies "Giulio Natta", National Research Council, Via Corti 12, 20133 Milan, Italy.

出版信息

Antioxidants (Basel). 2022 Aug 5;11(8):1526. doi: 10.3390/antiox11081526.

DOI:10.3390/antiox11081526
PMID:36009248
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9404846/
Abstract

Plants synthesize specific secondary metabolites for survival, reproduction, environmental resilience, and defense. Among them, lignans are a class of polyphenols with several bioactive properties: chemopreventive, anti-inflammatory, antiviral, and antioxidant. These compounds are often extracted from field-grown plants with very low yields. To overcome these constraints, in vitro tissue cultures provide a tool to optimize large-scale production. Moreover, the use of elicitation to increase secondary metabolite production is gaining importance. The aim of this work was to develop adventitious (ARL) and hairy roots (HRL) from , a species able to synthesize arylnaphthalene lignans such as justicidin B. The ARL and HRL were obtained for the first time and characterized for their phenol content, antioxidant activity, and the production of justicidin B after treatments with several elicitors and precursor feeding. Through NMR spectroscopy, other four lignans were highlighted and identified in the roots extracts. A pilot-scale bioreactor was adopted to assess the suitability of the developed root cultures for future large-scale production. The ARL and HRL cultures showed a justicidin B production higher than other species cultures described up to now (75.8 mg/L and 82.2 g/L), and the production more than doubled after elicitation with MeJA.

摘要

植物合成特定的次生代谢产物以实现生存、繁殖、环境适应和防御。其中，木脂素是一类具有多种生物活性的多酚：化学预防、抗炎、抗病毒和抗氧化。这些化合物通常从田间种植的植物中提取，产量极低。为了克服这些限制，体外组织培养提供了一种优化大规模生产的工具。此外，利用诱导来增加次生代谢产物的产量正变得越来越重要。这项工作的目的是从一种能够合成芳基萘木脂素（如异嗪皮啶B）的植物中诱导出不定根（ARL）和毛状根（HRL）。首次获得了ARL和HRL，并对其酚含量、抗氧化活性以及在用几种诱导剂处理和添加前体后异嗪皮啶B的产量进行了表征。通过核磁共振光谱，在根提取物中还发现并鉴定了其他四种木脂素。采用中试规模的生物反应器来评估所开发的根培养物用于未来大规模生产的适用性。ARL和HRL培养物中异嗪皮啶B的产量高于目前报道的其他物种培养物（分别为75.8毫克/升和82.2克/升），在用茉莉酸甲酯诱导后产量增加了一倍多。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71e4/9404846/a4b991f8f0c8/antioxidants-11-01526-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71e4/9404846/4a7adf051762/antioxidants-11-01526-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71e4/9404846/2a0437d22dee/antioxidants-11-01526-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71e4/9404846/e2743ae2b101/antioxidants-11-01526-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71e4/9404846/3421218929ca/antioxidants-11-01526-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71e4/9404846/07fe4afdab67/antioxidants-11-01526-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71e4/9404846/68c323c5110c/antioxidants-11-01526-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71e4/9404846/dc519bee3d71/antioxidants-11-01526-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71e4/9404846/0b04d397b470/antioxidants-11-01526-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71e4/9404846/dae4e581a188/antioxidants-11-01526-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71e4/9404846/a4b991f8f0c8/antioxidants-11-01526-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71e4/9404846/4a7adf051762/antioxidants-11-01526-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71e4/9404846/2a0437d22dee/antioxidants-11-01526-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71e4/9404846/e2743ae2b101/antioxidants-11-01526-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71e4/9404846/3421218929ca/antioxidants-11-01526-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71e4/9404846/07fe4afdab67/antioxidants-11-01526-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71e4/9404846/68c323c5110c/antioxidants-11-01526-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71e4/9404846/dc519bee3d71/antioxidants-11-01526-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71e4/9404846/0b04d397b470/antioxidants-11-01526-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71e4/9404846/dae4e581a188/antioxidants-11-01526-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71e4/9404846/a4b991f8f0c8/antioxidants-11-01526-g010.jpg

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