Institute of Chemical Sciences and Technologies "Giulio Natta", National Research Council, Via Mario Bianco 9, 20131 Milan, Italy.
Institute of Agricultural Biology and Biotechnology, National Research Council, Via Bassini 15, 20133 Milan, Italy.
Molecules. 2022 Apr 23;27(9):2732. doi: 10.3390/molecules27092732.
Lignans are plant phenols derived from phenylpropanoids. They play a significant role in plant defense and have features that make them appealing for pharmaceutical applications. Lignans can be obtained by plant in vitro cultures; their production by adventitious and hairy roots of species seems to be a promising alternative to chemical synthesis. In the context of large-scale production, it is necessary to optimize their extraction from plants tissue by choosing the more suitable solvent and extraction procedure, paying attention to the use of green media and methods. With the aim to select the best conditions for the extraction of two interesting lignans (justicidin B and 6-methoxypodophyllotoxin) from tissues, different green solvents and the method of ultrasound-assisted extraction were tested. The results showed that ethyl methyl ketone and dimethyl carbonate were the best media to extract justicidin B and 6-methoxypodophyllotoxin, respectively, in terms of purity and recovery. Moreover, we showed that ultrasound-assisted extraction presents different advantages compared to conventional methods. Finally, the optimal experimental conditions to extract justicidin B from hairy roots using methyl ethyl ketone were also determined by the response surface method. The models obtained are reliable and accurate to estimate the purity and recovery of justicidin B.
木脂素是源自苯丙烷类的植物酚类化合物。它们在植物防御中起着重要作用，并且具有使其在药物应用中具有吸引力的特征。木脂素可以通过植物的体外培养获得；其在 物种的不定根和毛状根中的产生似乎是化学合成的一种有前途的替代方法。在大规模生产的背景下，通过选择更合适的溶剂和提取程序，注意使用绿色介质和方法，从植物组织中优化其提取非常重要。本研究旨在选择从 组织中提取两种有趣的木脂素（冬凌草甲素和 6-甲氧基鬼臼毒素）的最佳条件，测试了不同的绿色溶剂和超声辅助提取方法。结果表明，就纯度和收率而言，乙基甲基酮和碳酸二甲酯分别是提取冬凌草甲素和 6-甲氧基鬼臼毒素的最佳介质。此外，我们还表明，超声辅助提取与常规方法相比具有不同的优势。最后，还通过响应面法确定了使用乙基甲基酮从 毛状根中提取冬凌草甲素的最佳实验条件。获得的模型可靠且准确，可以估计冬凌草甲素的纯度和回收率。
