Lewis Luján Lidianys María, Dörschmann Philipp, Seeba Charlotte, Thalenhorst Tabea, Roider Johann, Iloki Assanga Simon Bernard, Ruiz Juan Carlos Gálvez, Del Castillo Castro Teresa, Rosas-Burgos Ema Carina, Plascencia-Jatomea Maribel, Ezquerra Brauer Josafat Marina, Klettner Alexa
Departamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, Blvd. Luis Encinas y Rosales S/N, Col. Centro, Hermosillo 83000, Sonora, Mexico.
Department of Ophthalmology, University of Kiel, University Medical Center, Arnold-Heller-Str. 3, Haus 25, 24105 Kiel, Germany.
Antioxidants (Basel). 2022 Aug 15;11(8):1574. doi: 10.3390/antiox11081574.
Ommochromes are pigments of invertebrates that exhibit oxidative stress protection. The aim of this study was to investigate ommochromes extracted from cephalopod's skin for their ability to inhibit age-related-macular degeneration (AMD)-related factors such as HO-induced and iron-dependent oxidative stress (ferroptosis and erastin), accumulation of advanced glycation end-products (AGEs), as well as vascular endothelial growth factor (VEGF), and inflammatory cytokines (interleukin 6 and interleukin 8) secretion. As cell systems, we used primary porcine retinal pigment epithelium (RPE), human retinal pigment epithelium cell line ARPE-19 and uveal melanoma cell line OMM-1. In vitro, ommochromes produced an antiglycation effect by the inhibition of fructosylation reaction. The ommochromes showed protective effects against erastin- induced cell death in ARPE-19. In addition, in long-term stimulation (7 days) ommochromes decreased constitutively secreted VEGF, as well as interleukin 6 and interleukin 8 induced by Poly I:C in primary RPE. No relevant effects were detected in OMM-1 cells. The effects are dependent on the cell system, time of exposition, and concentration. This substance is of interest for further research concerning age-related macular degeneration.
眼色素是无脊椎动物的色素，具有氧化应激保护作用。本研究的目的是研究从头足类动物皮肤中提取的眼色素抑制年龄相关性黄斑变性（AMD）相关因子的能力，这些因子包括HO诱导的和铁依赖性氧化应激（铁死亡和埃拉斯汀）、晚期糖基化终产物（AGEs）的积累，以及血管内皮生长因子（VEGF）和炎性细胞因子（白细胞介素6和白细胞介素8）的分泌。作为细胞系统，我们使用了原代猪视网膜色素上皮（RPE）、人视网膜色素上皮细胞系ARPE-19和葡萄膜黑色素瘤细胞系OMM-1。在体外，眼色素通过抑制果糖基化反应产生抗糖化作用。眼色素对ARPE-19中埃拉斯汀诱导的细胞死亡具有保护作用。此外，在长期刺激（7天）中，眼色素降低了原代RPE中组成性分泌的VEGF以及Poly I:C诱导的白细胞介素6和白细胞介素8。在OMM-1细胞中未检测到相关作用。这些作用取决于细胞系统、暴露时间和浓度。这种物质对于年龄相关性黄斑变性的进一步研究具有重要意义。
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