Kato T, Murashima Y L
Neurochem Res. 1987 Jun;12(6):529-35. doi: 10.1007/BF01000237.
Freeze-dried sections (14 microns thick) were prepared from mice with normal (C57BL strain) and degenerated (C3H strain) retinas. GABA concentration and GAD activity were determined in the microsamples (1.8-20 ng dry weight) of retinal layers and sublayers, using an enzymatic amplication reaction, NADP cycling. GABA was distributed over all layers of normal retina with a broad concentration peak covering both inner nuclear and plexiform layers. In contrast, GAD activity was mostly localized in the inner plexiform layer. GABA concentration was similar in one-fourth of the sublayers of each inner nuclear or plexiform layer. GAD activity was highest in the innermost sublayer of the inner nuclear layer. An increasing gradient of GAD activity was present in the inward direction in the inner plexiform layer. In the degenerated retina, lacking in photoreceptors, the inner nuclear and plexiform layers remained, and GABA and GAD levels in these layers were similar to those in normal retina.
从具有正常（C57BL品系）和退化（C3H品系）视网膜的小鼠制备冷冻干燥切片（14微米厚）。使用酶促扩增反应、NADP循环，测定视网膜各层和亚层的微量样本（干重1.8 - 20纳克）中的GABA浓度和GAD活性。GABA分布于正常视网膜的所有层，在内核层和神经丛层有一个较宽的浓度峰值。相比之下，GAD活性主要定位于内神经丛层。每个内核层或神经丛层四分之一的亚层中GABA浓度相似。GAD活性在内核层最内层的亚层中最高。在内神经丛层中，GAD活性向内呈递增梯度。在缺乏光感受器的退化视网膜中，内核层和神经丛层仍然存在，这些层中的GABA和GAD水平与正常视网膜中的相似。
