Sarthy P V
J Neurosci Methods. 1982 Jan;5(1-2):77-82. doi: 10.1016/0165-0270(82)90054-1.
We have examined the uptake of [3H]GABA by isolated glial (Müller) cells using light microscope autoradiography. The aim of these studies was to determine whether Müller cells dissociated from papain-treated mouse retina have the capacity to accumulate exogenous GABA and to further characterize the uptake process. Our results show that isolated Müller cells retain the capacity to accumulate exogenous [3H]GABA and this uptake is inhibited by excess GABA, DABA and beta-alanine. This communication also describes a new method for performing light microscope autoradiography.
我们使用光学显微镜放射自显影技术检测了分离的神经胶质（米勒）细胞对[3H]GABA的摄取。这些研究的目的是确定从木瓜蛋白酶处理的小鼠视网膜分离出的米勒细胞是否有能力积累外源性GABA，并进一步表征摄取过程。我们的结果表明，分离的米勒细胞保留了积累外源性[3H]GABA的能力，并且这种摄取受到过量GABA、DABA和β-丙氨酸的抑制。本通讯还描述了一种进行光学显微镜放射自显影的新方法。
