Schnitzer J, Rusoff A C
J Neurosci. 1984 Dec;4(12):2948-55. doi: 10.1523/JNEUROSCI.04-12-02948.1984.
We have used an antiserum to L-glutamic acid decarboxylase ((GAD), a synthesizing enzyme for gamma-aminobutyric acid (GABA)) to localize putative GABAergic neurons in the developing C57BL/6J mouse retina. At early developmental stages (embryonic day 17 to postnatal day 3), strong GAD-like immunoreactivity is detectable in cell bodies located within the neuroblastic layer. These cells have relatively large cell bodies and extend several sturdy processes which are oriented radially at these early stages. We have identified these cells as horizontal cells. In addition, cell bodies adjacent to the inner plexiform layer and both diffuse and punctate structures within the inner plexiform layer proper have weak GAD-like immunoreactivity at this time. By postnatal day 6, GAD-positive horizontal cell processes begin to form a horizontal network in the newly formed outer plexiform layer. Immunolabeling of amacrine cell bodies and of punctate structures in the inner plexiform layer becomes much stronger at this time, reaching a maximum staining intensity during the second postnatal week. After postnatal day 12, GAD-like immunoreactivity of the horizontal cells begins to decline; in 4-week-old mice the horizontal cells are no longer detectably labeled by this GAD antiserum. At the same time, the GAD-like-immunoreactive material in the inner plexiform layer becomes stratified, forming distinct layers. Amacrine cells and the inner plexiform layer remain GAD positive into adulthood.
我们使用了一种针对L-谷氨酸脱羧酶（（GAD），γ-氨基丁酸（GABA）的合成酶）的抗血清，来定位发育中的C57BL/6J小鼠视网膜中假定的GABA能神经元。在发育早期阶段（胚胎第17天至出生后第3天），在位于神经母细胞层内的细胞体中可检测到强烈的GAD样免疫反应性。这些细胞具有相对较大的细胞体，并伸出几个粗壮的突起，在这些早期阶段呈放射状排列。我们已将这些细胞鉴定为水平细胞。此外，此时与内网状层相邻的细胞体以及内网状层本身内的弥漫性和点状结构具有较弱的GAD样免疫反应性。到出生后第6天，GAD阳性的水平细胞突起开始在新形成的外网状层中形成水平网络。此时，无长突细胞体和内网状层中点状结构的免疫标记变得更强，在出生后第二周达到最大染色强度。出生后第12天之后，水平细胞的GAD样免疫反应性开始下降；在4周龄的小鼠中，水平细胞不再能用这种GAD抗血清检测到标记。与此同时，内网状层中的GAD样免疫反应性物质分层，形成不同的层。无长突细胞和内网状层在成年期仍保持GAD阳性。
